Food Science and Biotechnology

, Volume 25, Issue 6, pp 1753–1760 | Cite as

Long-chain bases from Cucumaria frondosa inhibit adipogenesis and regulate lipid metabolism in 3T3-L1 adipocytes

  • Yingying Tian
  • Shiwei HuEmail author
  • Hui Xu
  • Jingfeng Wang
  • Changhu Xue
  • Yuming Wang


This study aims to investigate anti-adipogenic effects of long-chain bases from Cucumaria frondosa (Cf-LCBs) in vitro. Results showed that Cf-LCBs inhibited adipocyte differentiation and the expressions of CCAAT/enhancer binding proteins (C/EBPs) and peroxisome proliferators-activated receptor γ (PPARγ). Cf-LCBs increased β-catenin mRNA and nuclear translocation and increased its target genes, cyclin D1 and c-myc. Cf-LCBs enhanced fizzled and lipoprotein-receptor-related protein5/6 (LRP5/6) expressions, whereas wingless-type MMTV integration site10b (WNT10b) and glycogen syntheses kinase 3β (GSK3β) remained unchanged. Cf-LCBs also reduced adipogenesis and recovered WNT/β-catenin signaling in the cells suffering from 21H7, a β-catenin inhibitor. In addition, Cf-LCBs decreased triglyceride content and the expressions of lipogenesis genes. Cf-LCBs increased FFA levels and the expressions of lipidolytic factors. Cf-LCBs promoted the phosphorylation of adenosine-monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase. These findings indicate that Cf-LCBs inhibit adipogenesis through activation of WNT/β-catenin signaling and regulate lipid metabolism via activation of AMPK pathway.


Cusumaria frondosa long-chain bases adipocyte differentiation lipid metabolism 


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Yingying Tian
    • 1
    • 2
  • Shiwei Hu
    • 1
    • 2
    Email author
  • Hui Xu
    • 2
  • Jingfeng Wang
    • 2
  • Changhu Xue
    • 2
  • Yuming Wang
    • 2
  1. 1.Innovation Application InstituteZhejiang Ocean UniversityZhoushan, ZhejiangChina
  2. 2.College of Food Science and EngineeringOcean University of ChinaQingdao, ShandongChina

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