Food Science and Biotechnology

, Volume 25, Issue 2, pp 623–629 | Cite as

Compositional analysis of walnut lipid extracts and properties as an anti-cancer stem cell regulator via suppression of the self-renewal capacity

  • Jooyeon Chung
  • Yoo-Sun Kim
  • Jisoo Lee
  • Jae Hwan Lee
  • Sang-Woon Choi
  • Yuri Kim


Colon cancer is a leading cause of cancer-related deaths worldwide. Effects of walnut (Juglans regia L.) lipid extracts (WLEs) on the self-renewal capacity of cancer stem cells (CSCs) in colon cancer were investigated. The dominant component of WLEs was α-linoleic acid (64.6%), followed by α-linolenic acid (14.6%), and oleic acid (12.6%). A higher concentration of γ-tocopherol (37.1%) was also present than of α-tocopherol (0.6%). CD133+CD44+CSCs treated with WLEs showed inhibition of colony formation and sphere formation, indicating a decrease in the self-renewal capacity. Treatment with WLEs also resulted in down-regulation of protein levels, including Notch1, phospho-GSK3β (p-GSK3β), and β-catenin, which are associated with CSCs and the self-renewing capacity. WLEs rich in essential fatty acids and γ-tocopherol can exert therapeutic actions on colon cancer via targeting of CSCs.


walnut lipid extract colon cancer cancer stem cell self-renewal capacity 


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jooyeon Chung
    • 1
  • Yoo-Sun Kim
    • 1
  • Jisoo Lee
    • 1
  • Jae Hwan Lee
    • 2
  • Sang-Woon Choi
    • 3
  • Yuri Kim
    • 1
  1. 1.Department of Nutritional Science and Food ManagementEwha Womans UniversitySeoulKorea
  2. 2.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonKorea
  3. 3.Chaum Life CenterCHA UniversitySeoulKorea

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