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Food Science and Biotechnology

, Volume 25, Issue 2, pp 525–532 | Cite as

Antimicrobial activity of Bacillus amyloliquefaciens EMD17 isolated from Cheonggukjang and potential use as a starter for fermented soy foods

  • Jae Yong Lee
  • Jae Min Shim
  • Zhuang Yao
  • Xiaoming Liu
  • Kang Wook Lee
  • Hyun-Jin Kim
  • Kyung-Sik Ham
  • Jeong Hwan KimEmail author
Article

Abstract

A Bacillus strain with antimicrobial activity was isolated from Cheonggukjang and identified as Bacillus amyloliquefaciens. Filtered culture supernatants of B. amyloliquefaciens EMD17 strongly inhibited growth of Bacillus cereus ATCC14579, Listeria monocytogenes ATCC19111, and an ochratoxin (OTA) producing Penicillium spp. The antimicrobial activity was not decreased by heat, pH, and proteases treatment, indicating a non-proteinous nature of the antimicrobial substance. A B. amyloliquefaciens EMD17 culture added to a B. cereus ATCC14579 culture killed B. cereus cells completely in 6 h, showing the bacteriocidal effect. The srfAA gene encoding surfactin synthetase A was detected using PCR, indicating that surfactin might be the responsible agent. Genes encoding fengycin and iturin were also detected. MS indicated production of lipopeptides, including surfactin. Cheonggukjang and Doenjang were prepared using B. amyloliquefaciens EMD17 as a starter and growth of spiked B. cereus cells was completely inhibited.

Keywords

Bacillus amyloliquefaciens antimicrobial activity lipopeptide surfactin starter 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jae Yong Lee
    • 1
  • Jae Min Shim
    • 1
  • Zhuang Yao
    • 1
  • Xiaoming Liu
    • 1
  • Kang Wook Lee
    • 1
  • Hyun-Jin Kim
    • 1
    • 3
  • Kyung-Sik Ham
    • 2
  • Jeong Hwan Kim
    • 1
    • 3
    Email author
  1. 1.Division of Applied Life Science (BK21 plus), Graduate SchoolGyeongsang National UniversityJinju, GyeongnamKorea
  2. 2.Department of Food Engineering and Solar Salt Research CenterMokpo National UniversityMuan, JeonnamKorea
  3. 3.Institute of Agriculture and Life ScienceGyeongsang National UniversityJinju, GyeongnamKorea

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