Effects of a chitosan coating on properties of retinol-encapsulated zein nanoparticles

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Zein nanoparticles coated with chitosan were prepared for encapsulation of retinol. The mean particle size of retinol-loaded zein nanoparticles was approximately 300 nm. After coating with chiotsan, the mean particle size increased to >500 nm. The zeta potential of retinol-loaded zein nanoparticles was approximately -30 mV, which was changed to >+24 mV after chitosan coating. The encapsulation efficiency of zein nanoparticles increased from 64.9% to >80% after chitosan coating. The release profile was greatly improved with a chitosan coating, and a slow release rate was observed. FTIR analysis of retinol-loaded chitosan-coated zein nanoaprticles showed cross-linking between zein and retinol through electrostatic interactions. Photochemical stability against UV light was greatly improved to >90% after encapsulation. The concentration of chitosan, the Mw of chitosan, and the zein/chitosan volume ratio were important parameters affecting the properties of nanoparticles.

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  1. 1.

    Guénin EP, Zatz JL. Skin permeation of retinylpalmitate from vescicles. J. Soc. Cosmet. Chem. 46: 261–270 (1995)

  2. 2.

    Varani J, Warner RL, Gharaee-Kermani M, Phan SH, Kang S, Chung JH, Wang ZQ, Datta SC, Fisher GJ, Voorhees JJ. Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin. J. Invest. Dermatol. 114: 480–486 (2000)

  3. 3.

    Tsunoda T, Takabashy K. Stability of all-trans-retinol in cream. J. Soc. Cosmet. Chem. 46: 191–198 (1995)

  4. 4.

    Vahlquist A. What are natural retinoids? J. Dermatol. 199: 3–11 (1999)

  5. 5.

    Andersson E, Rosdahl I, Trm H, Vahlquist A. Ultraviolet irradiation depletes cellular retinol and alters the metabolism of retinoic acid in cultured human keratinocytes and melanocytes. Melanoma Res. 3: 339–346 (1999)

  6. 6.

    Sorg O, Tan C, Carraux P, Didierjean L, Saurat JH. Retinol and retinyl esters epidermal pools are not identically sensitive to UVB irradiation and antioxidant protective effect. J. Dermatol. 199: 302–307 (1999)

  7. 7.

    Jenning V, Schafer-Korting M, Gohla S. Vitamin A-loaded solid lipid nanoparticles for topical use: Drug release properties. J. Control. Release 66: 115–126 (2000)

  8. 8.

    Kim DG, Jeong YI, Choi CY, Roh SH, Kang SK, Jang MK, Nah JW. Retinolencapsulated low molecular water-soluble chitosan nanoparticles. Int. J. Pharm. 391: 130–138 (2006)

  9. 9.

    Chen L, Remondetto GE, Subirade M. Food protein-based materials as nutraceutical delivery systems. Trends Food Sci. Tech. 17: 272–283 (2006)

  10. 10.

    Lai LF, Guo HX. Preparation of new 5-fluorouracil-loaded zein nanoparticles for liver targeting. Int. J. Pharm. 404: 317–323 (2011)

  11. 11.

    Luo Y, Zhang B, Whent M, Yu L, Wang Q. Preparation and characterization of zein/chitosan complex for encapsulation of a-tocopherol, and its in vitro controlled release study. Colloid. Surface B 85: 145–152 (2011)

  12. 12.

    Shukla R, Cheryan M. Zein: The industrial protein from corn. Ind. Crop. Prod. 13: 171–192 (2001)

  13. 13.

    Muthuselvi L, Dhathathreyan A. Simple coacervates of zein to encapsulate gitoxin. Colloid. Surface B 85: 39–43 (2006)

  14. 14.

    Fan W, Yan W, Xu ZS, Ni H. Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic technique. Colloid. Surface B 90: 21–27 (2012)

  15. 15.

    Luo Y, Teng Z, Wang Q. Development of zein nanoparticles coated with carboxymethyl chitosan for encapsulation and controlled release of vitamin D3. J. Agr. Food Chem. 60: 836–843 (2012)

  16. 16.

    Shim YS, Kim KJ, Seo DW. Rapid method for the determination of vitamins A and E in food using ultra-high-performance liquid chromatography. J. AOAC Int. 95: 517–522 (2012)

  17. 17.

    Kim BK, Lee JS, Oh JK, Park DJ. Preparation of resveratol-loaded poly (ε-caprolactone) nanoparticles by oil-in-water emulsion solvent evaporation method. Food Sci. Biotechnol. 18: 157–161 (2009)

  18. 18.

    Liu N, Park HJ. Chitosan-coated nanoliposome as vitamin E carrier. J. Microencapsul. 26: 235–242 (2009)

  19. 19.

    Shah S, Pal A, Kaushik VK, Devi S. Preparation and characterization of venlaxine hydrochloride-loaded chitosan nanoparticles and in vitro release of drug. J. Appl. Polym. Sci. 112: 2876–2887 (2009)

  20. 20.

    Gan Q, Wang T, Cochrane C, McCarron P. Modulation of surface, particle size and morphological properties of chitosan-TPP nanoparticles intended for gene delivery. Colloid. Surface B 44: 65–73 (2005)

  21. 21.

    Gan Q, Wang T. Chitosan nanoparticle as protein delivery carrier-systematic examination of fabrication conditions for efficient loading and release. Colloid. Surface B 59: 24–34 (2007)

  22. 22.

    Vandenberg GW, Drolet C, Scott SL, Noue JD. Factors affecting protein release from alginate-chitosan coacervate microcapsules during production and gastric/intestinal simulation. J. Control. Release 77: 297–307 (2011)

  23. 23.

    Cho AR, Chun YK, Kim BK, Park DJ. Preparation of chitosan-TPP microspheres as resveratrol carriers. J. Food Sci. 79: 568–576 (2014)

  24. 24.

    Luo Y, Zhang B, Cheng WH, Wang Q. Preparation, characterization and evaluation of selenite-loaded chitosan/TPP nanoparticles with or without zein coating. Carbohyd. Polym. 82: 942–951 (2010)

  25. 25.

    Desai KGH, Liu C, Park HJ. Characteristics of vitamin C encapsulated tripolyphosphate chitosan microspheres as affected by chitosan molecular weight. J. Microencapsul. 23: 79–90 (2006)

  26. 26.

    Antoniou J, Liu F, Majeed H, Qi J, Yokoyama W, Zhong F. Physicochmical and morphological properties of size-controlled chitosan-tripolyphosphate nanoparticles. Colloid. Surface A 465: 137–146 (2015)

  27. 27.

    Hu B, Pan CL, Hou ZY, Ye H, Hu B, Zeng XX. Optimization of fabrication parameters to produce chitosan-tripolyphosphate nanoparticles for delivery of tea catechins. J. Agr. Food Chem. 56: 7451–7458 (2008)

  28. 28.

    Krauland AH, Alonso MJ. Chitosan/cyclodextrin nanoparticles as macromolecular drug delivery system. Int. J. Pharm. 340: 134–142 (2007)

  29. 29.

    Li XY, Kong XY, Shi S, Zheng XL, Guo G, Wei YQ, Qian ZY. Preparation of alginate coated chitosan microparticles for vaccine delivery. BMC Biotechnol. 8: 1–11 (2008)

  30. 30.

    Jarudilokkul S, Tongthammachat A, Boonamnuayvittaya V. Preparation of chitosan nanoparticles for encapsulation and release of protein. Korean J. Chem. Eng. 28: 1247–1251 (2011)

  31. 31.

    Kim TH, Park YH, Kim KJ, Cho CS. Release of albumin from chitosan-coated pectin beads in vitro. Int. J. Pharm. 250: 371–383 (2003)

  32. 32.

    Jee JP, Lim SJ, Park JS, Kim CK. Stabilization of all-trans retinol by loading lipophilic antioxidants in solid lipid nanoparticles. Eur. J. pharm. Biopharm. 63: 134–139 (2006)

  33. 33.

    Sessa DJ, Mohamed A, Byars JA. Chemistry and physical properties of meltprocessed and solution-cross-linked corn zein. J. Agr. Food Chem. 56: 7067–7075 (2008)

  34. 34.

    Youan BBC, Jackson TL, Dickens L, Hernandez C, Ababio GO. Protein release profiles and morphology of biodegradable microcapsules containing an oily core. J. Control. Release 73: 313–326 (2001)

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Correspondence to Bum-Keun Kim.

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Park, C., Park, D. & Kim, B. Effects of a chitosan coating on properties of retinol-encapsulated zein nanoparticles. Food Sci Biotechnol 24, 1725–1733 (2015).

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  • retinol
  • zein
  • chitosan
  • nanoparticle
  • encapsulation