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Food Science and Biotechnology

, Volume 24, Issue 1, pp 37–40 | Cite as

Presence of β-amylase in ramie leaf and its anti-staling effect on rice cake

  • Dang Hai Dang Nguyen
  • Phuong Lan Tran
  • Hyun Sook Ha
  • Jin Sil Lee
  • Wan Soo Hong
  • Quang Tri Le
  • Byung Chul Oh
  • Sung Hoon ParkEmail author
Research Article

Abstract

Presence of β-amylase in ramie leaf and its anti-staling effect on starch-based foods were assessed. The ammonium sulfate fractionate (80% saturation) of the ramie leaf extracts showed a β-amylase activity, giving maltose (Glc2) as a major product, exclusively, when incubating with maltopentaose (Glc5) or soluble starch at 45°C, pH 6.0. The starch-based food product (rice cake) prepared with freeze-dried ramie leaf enzyme revealed that the linear maltooligosaccharides ranging from Glc2 to Glc6 significantly increased and the shorter branch chains (DP<15) of amylopectin increased whereas the longer branch chains (DP>16) decreased in the product. These results demonstrated that maltosyl residue was released from the non-reducing end of the longer branch chains of amylopectin by β-amylase. The ramie leaf-treatment sample significantly reduced the retrogradation rate during 48 h storage at 4°C. As an alternative plant-origin enzyme, the ramie leaf β-amylase has potential for a novel anti-staling additive.

Keywords

ramie leaf plant-origin β-amylase amylopectin starch-based food anti-staling additive 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Dang Hai Dang Nguyen
    • 1
  • Phuong Lan Tran
    • 1
  • Hyun Sook Ha
    • 1
  • Jin Sil Lee
    • 1
  • Wan Soo Hong
    • 1
  • Quang Tri Le
    • 2
    • 3
  • Byung Chul Oh
    • 4
  • Sung Hoon Park
    • 4
    Email author
  1. 1.Department of Food Service Management and NutritionSangmyung UniversitySeoulKorea
  2. 2.Faculty of Food TechnologySaiGon Technology UniversityHo Chi Minh CityVietNam
  3. 3.TienGiang Department of Scinece and TechnologyMyTho City, Tien Giang ProvinceVietNam
  4. 4.Lee Gil Ya Cancer and Diabetes InstituteGachon University of Medicine and ScienceIncheonKorea

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