Physicochemical properties and antioxidant activities of melanin and fractions from Auricularia auricula fruiting bodies
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Fractions I and II from Auricularia auricula fruiting body (AAFB) melanin were separated using Sephadex G-100 column chromatography and their physicochemical properties and antioxidant activities were investigated. Molecular mass values of the fractions were 384 and 47 kDa, respectively. Fraction I exhibited lower L*, a*, and b* values than AAFB melanin and fraction II. AAFB melanin and fractions possessed the same solubility and spectroscopic properties, which were similar to the properties of other melanin. Elemental composition analysis revealed that the main component of AAFB melanin and fractions was pheomelanin. AAFB melanin and fractions, particularly fraction II, exhibited strong antioxidant activities based on Fe2+-chelating, DPPH radical scavenging, and superoxide radical scavenging activities.
KeywordsAuricularia auricula melanin fraction property antioxidant activity
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