Molecular cloning and expression of amylosucrase from highly radiation-resistant Deinococcus radiopugnans
Research Note
First Online:
Received:
Revised:
Accepted:
- 120 Downloads
- 4 Citations
Abstract
Amylosucrase (AS), a glucosyltransferase of the glycoside hydrolase 13 family, does not require a nucleotide-activated sugar as a glucosyl-donor. A gene (drpas) of Deinococcus radiopugnans encoding a putative AS was identified and cloned for characterization. The amino acid sequence revealed that drpas exhibited 76 and 74% identities with AS genes from D. geothermalis and D. radiodurans, respectively. Recombinant DRpAS exhibited AS activities. The ratios of hydrolysis, polymerization, and isomerization reactions were 5.8:82.7:11.5 at 40°C. The DRpAS was highly thermostable and produced more polymerization products than AS from D. radiodurans although the optimum growth temperature of both strains is 30°C.
Keywords
amylosucrase Deinococcus radiopugnans glycoside hydrolase 13 family (GH13) insoluble glucan transglycosylationPreview
Unable to display preview. Download preview PDF.
References
- 1.Seo DH, Jung JH, Ha SJ, Yoo SH, Kim TJ, Cha J, Park CS. Molecular Cloning of the Amylosucrase Gene from a Moderate Thermophilic Bacterium Deinococcus geothermalis and Analysis of its Dual Enzyme Activity. Woodhead Publiching Limited, Cambridge, UK. pp. 125–140 (2008)Google Scholar
- 2.Champion E, André I, Moulis C, Boutet J, Descroix K, Morel S, Monsan P, Mulard LA, Remaud Siméon M. Design of α-transglucosidases of controlled specificity for programmed chemoenzymatic synthesis of antigenic oligosaccharides. J. Am. Chem. Soc. 131: 7379–7389 (2009)CrossRefGoogle Scholar
- 3.Ryu JH, Lee BH, Seo DH, Baik MY, Park CS, Wang R, Yoo SH. Production and characterization of digestionresistant starch by the reaction of Neisseria polysaccharea amylosucrase. Starch-Starke 62: 221–228 (2010)CrossRefGoogle Scholar
- 4.Wang R, Bae JS, Kim JH, Kim BS, Yoon SH, Park CS, Yoo SH. Development of an efficient bioprocess for turanose production by sucrose isomerisation reaction of amylosucrase. Food Chem. 132: 773–779 (2012)CrossRefGoogle Scholar
- 5.Albenne C, Skov LK, Tran V, Gajhede M, Monsan P, Remaud Siméon M, André Leroux G. Towards the molecular understanding of glycogen elongation by amylosucrase. Proteins 66: 118–126 (2007)CrossRefGoogle Scholar
- 6.Ha SJ, Seo DH, Jung JH, Cha J, Kim TJ, Kim YW, Park CS. Molecular cloning and functional expression of a new amylosucrase from Alteromonas macleodii. Biosci. Biotech. Bioch. 73: 1505–1512 (2009)CrossRefGoogle Scholar
- 7.Seo DH, Choi HC, Kim HH, Yoo SH, Park CS. Functional expression of amylosucrase, a glucan-synthesizing enzyme, from Arthrobacter chlorophenolicus A6. J. Microbiol. Biotechn. 22: 1253–1257 (2012)CrossRefGoogle Scholar
- 8.Pizzut Serin S, Potocki Véronèse G, van der Veen BA, Albenne C, Monsan P, Remaud Simeon M. Characterisation of a novel amylosucrase from Deinococcus radiodurans. FEBS lett. 579: 1405–1410 (2005)CrossRefGoogle Scholar
- 9.Jeong JW, Seo DH, Jung JH, Park JH, Baek NI, Kim MJ, Park CS. Biosynthesis of glucosyl glycerol, a compatible solute, using intermolecular transglycosylation activity of amylosucrase from Methylobacillus flagellatus KT. Appl. Biochem. Biotech. 173: 904–917 (2014)CrossRefGoogle Scholar
- 10.De Montalk GP, Remaud Simeon M, Willemot R, Planchot V, Monsan P. Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme. J. Bacteriol. 181: 375–381 (1999)Google Scholar
- 11.Skov LK, Mirza O, Sprogøe D, Dar I, Remaud Simeon M, Albenne C, Monsan P, Gajhede M. Oligosaccharide and sucrose complexes of amylosucrase structural implications for the polymerase activity. J. Biol. Chem. 277: 47741–47747 (2002)CrossRefGoogle Scholar
- 12.Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M. The RAST Server: Rapid annotations using subsystems technology. BMC Genomics 9:75 (2008)CrossRefGoogle Scholar
- 13.Champion E, Gueìrin Fdr, Moulis C, Barbe S, Tran TH, Morel S, Descroix K, Monsan P, Mourey L, Mulard LA. Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides. J. Am. Chem. Soc. 134: 18677–18688 (2012)CrossRefGoogle Scholar
- 14.Filipkowski P, Koziatek M, Kur J. A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans. Extremophiles 10: 607–614 (2006)CrossRefGoogle Scholar
- 15.Albenne C, Skov LK, Mirza O, Gajhede M, Feller G, D’Amico S, André G, Potocki Véronèse G, van der Veen BA, Monsan P. Molecular basis of the amylose-like polymer formation catalyzed by Neisseria polysaccharea amylosucrase. J. Biol. Chem. 279: 726–734 (2004)CrossRefGoogle Scholar
Copyright information
© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2014