Food Science and Biotechnology

, Volume 22, Issue 6, pp 1481–1486 | Cite as

Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD

  • Hyun Cheol Jeong
  • You-Shin Shim
  • Young Kyoung Rhee
  • Sang Yoon Choi
  • Hee-Do Hong
  • Jayong Chung
  • Myung Joo Han
  • Chang-Won Cho
Research Article

Abstract

An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R2 >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 μg/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 μg/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06–105.81% with an RSD value of 1.87–3.50%. In conclusion, the results of validation showed that the proposed HPLC method was useful for the quantification of the two marker compounds in C. setidens.

Keywords

Cirsium setidens Nakai marker compound flavone HPLC-DAD validation 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hyun Cheol Jeong
    • 1
  • You-Shin Shim
    • 1
  • Young Kyoung Rhee
    • 1
  • Sang Yoon Choi
    • 1
  • Hee-Do Hong
    • 1
  • Jayong Chung
    • 2
  • Myung Joo Han
    • 2
  • Chang-Won Cho
    • 1
  1. 1.Korea Food Research InstituteGyeonggiKorea
  2. 2.Department of Food and NutritionKyung Hee UniversitySeoulKorea

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