Food Science and Biotechnology

, Volume 22, Issue 3, pp 845–851 | Cite as

Acteoside inhibits irradiation-mediated decreases in the viability and DNA synthesis of MC3T3-E1 cells

  • Kyoung-A. Kim
  • Seung-Ah Lee
  • Ki-Hyun Kim
  • Keun-Soo Lee
  • Jeong-Chae Lee
Research Article
First Online:
Received:
Revised:
Accepted:

Abstract

Therapeutic irradiation can cause bone loss, whereas antioxidant supplementation is considered to attenuate irradiation-mediated damages. This study examined whether or not acteoside inhibits irradiation-mediated changes in viability and proliferation of MC3T3-E1 cells. X-ray radiation at >4 Gy not only decreased cell viability and DNA synthesis in the cells, but also increased intracellular levels of reactive oxygen species (ROS) and phosphorylated p66Shc protein. Irradiation at 8Gy also decreased intracellular levels of reduced glutathione (GSH) and induced G1 phase arrest of cell cycle progression with the attendant increase of p21 induction. Pretreatment with acteoside inhibited the irradiation-mediated decreases in viability and DNA synthesis by restoring the radiation-mediated changes in the levels of ROS, GSH, p21, and p-p66Shc to the untreated control levels. These inhibitory activities of acteoside were greater than that of a synthetic antioxidant compound or N-acetyl cysteine did. Collectively, acteoside treatment may prevent irradiation-induced oxidative damages to osteoblasts.

Keywords

X-ray radiation MC3T3-E1 cell oxidative damage acteoside viability 
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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kyoung-A. Kim
    • 1
  • Seung-Ah Lee
    • 2
  • Ki-Hyun Kim
    • 3
  • Keun-Soo Lee
    • 4
  • Jeong-Chae Lee
    • 3
    • 5
  1. 1.Department of Oral and Maxillofacial Radiology and School of Dentistry, Research Institute of Clinical MedicineChonbuk National UniversityJeonju, JeonbukKorea
  2. 2.Department of NursingChonnam Techno CollegeJeonju, JeonbukKorea
  3. 3.Department of Orthodontics and School of DentistryChonbuk National UniversityJeonju, JeonbukKorea
  4. 4.Research Laboratory, RM317 Acetechno Tower-9Korea Bone Bank Co., Ltd.SeoulKorea
  5. 5.Research Center of Bioactive MaterialsChonbuk National UniversityJeonju, JeonbukKorea

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