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Occurrence of biogenic amines in Doubanjiang and Tofu

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Abstract

This study was performed to analyze biogenic amine contents and other parameters in doubanjiang and tofu. Through this study, it was found that doubanjiang contained considerably large amounts of most biogenic amines (>30 mg/kg of β-phenylethylamine in particular), and tofu had a relatively high level of spermidine (>20 mg/ kg). Therefore, the amounts of biogenic amines in the foods seem to be occasionally beyond the safe level for human consumption. Meanwhile, the biogenic amine contents in doubanjiang showed a good relationship with salt content (R2=0.89). The spermidine content in tofu samples was closely related to that in soybean, the raw material of tofu. There also appeared to be a good relationship (R2=0.82) between the biogenic amine contents and total plate counts in doubanjiang, but not in tofu. Most strains from the foods were capable of producing biogenic amines, and the identification revealed that bacterial ability to produce biogenic amines was determined at the level of strains rather than species. Taken together, it seems that biogenic amine contents in doubanjiang are mainly affected by fermentation processes, whereas those in tofu are primarily affected by raw materials.

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References

  1. Whitaker JR. Biochemical changes occurring during the fermentation of high-protein foods. Food Technol. -Chicago 32: 175–180 (1978)

    CAS  Google Scholar 

  2. Han B-Z, Rombouts FM, Nout MJR. A Chinese fermented soybean food. Int. J. Food Microbiol. 65: 1–10 (2001)

    Article  CAS  Google Scholar 

  3. Wang HL, Hesseltine CW. Sufu and lao-chao. J. Agr. Food Chem. 18: 572–575 (1970)

    Article  CAS  Google Scholar 

  4. Brink B, Damirik C, Joosten HMLJ, Huis in’t Veld JHJ. Occurrence and formation of biologically active amines in foods. Int. J. Food Microbiol. 11: 73–84 (1990)

    Article  Google Scholar 

  5. Halász A, Baráth Á, Simon-Sarkadi L, Holzapfel W. Biogenic amines and their production by microorganisms in food. Trends Food Sci. Tech. 5: 42–49 (1994)

    Article  Google Scholar 

  6. Rice SL, Eitenmiller RR, Koehler PE. Biologically active amines in foods: A review. J. Milk Food Technol. 39: 353–358 (1976)

    CAS  Google Scholar 

  7. Askar A, Treptow H. Biogene amine in lebensmitteln (Biogenic amines in agri-food products). pp. 21–74. In: Vorkommen, Bedeutung und Bestimmung (Occurrence, Significance, and Determination). Askar A, Treptow H (eds). Verlag Eugen Elmer, Stuttgart, Germany (1986)

    Google Scholar 

  8. Joosten HMLJ, Nuñez M. Prevention of histamine formation in cheese by bacteriocin-producing lactic acid bacteria. Appl. Environ. Microb. 62: 1178–1181 (1996)

    CAS  Google Scholar 

  9. Taylor SL. Histamine food poisoning: Toxicology and clinical aspects. Crit. Rev. Toxicol. 17: 91–128 (1986)

    Article  CAS  Google Scholar 

  10. Huis In’t Veld JHJ, Hose H, Schaafsma GJ, Silla H, Smith JE. Health aspects of food biotechnology. Vol. 2, pp. 2.73–2.97. In: Processing and Quality of Foods. Food Biotechnology: Avenues to Healthy and Nutritious Products. Zeuthen P, Cheftel JC, Ericksson C, Gormley TR, Link P, Paulus K (eds). Elsevier Applied Science, London, UK (1990)

    Google Scholar 

  11. Chin KDH, Koehler PE. Identification and estimation of histamine, tryptamine, phenylethylamine, and tyramine in soy sauce by thin layer chromatography of dansyl derivatives. J. Food Sci. 48: 1826–1828 (1983)

    Article  CAS  Google Scholar 

  12. Mower HF, Bhagavan NV. Tyramine content of Asian and Pacific foods determined by high performance liquid chromatography. Food Chem. 31: 251–257 (1989)

    Article  CAS  Google Scholar 

  13. Nout MJR, Ruiker MMW, Bouwmeester HM. Effect of processing conditions on the formation of biogenic amines and ethyl carbanate in soybean tempe. J. Food Safety 13: 293–303 (1993)

    Article  CAS  Google Scholar 

  14. Stratton JE, Taylor SL. Scombroid poisoning. pp. 331–351. In: Microbiology of Marine Food Products. Ward DR, Hackney CR (eds). Van Nostrand Reinhold Co., New York, NY, USA (1991)

    Chapter  Google Scholar 

  15. AOAC. Official Methods of Analysis of AOAC Int. 16th ed. Method 925.55. Association of Official Analytical Chemists International, Arlington, VA, USA (1995)

    Google Scholar 

  16. Ben-Gigirey B, De Sousa JMVB, Villa TG, Barros-Velazquez J. Changes in biogenic amines and microbiological analysis in albacore (Thunnus alalunga) muscle during frozen storage. J. Food Protect. 61: 608–615 (1998)

    CAS  Google Scholar 

  17. Eerola S, Hinkkanen R, Lindfors E, Hirvi T. Liquid chromatographic determination of biogenic amines in dry sausages. J. AOAC Int. 76: 575–577 (1993)

    CAS  Google Scholar 

  18. Ben-Gigirey B, De Sousa JMVB, Villa TG, Barros-Velazquez J. Histamine and cadaverine production by bacteria isolated from fresh and frozen albacore (Thunnus alalunga). J. Food Protect. 62: 933–939 (1999)

    CAS  Google Scholar 

  19. Claus D, Berkeley RCW. Genus Bacillus. Vol. 2, pp. 1105–1139. In: Bergey’s Manual of Systematic Bacteriology. Sneath HAP (ed). Williams & Wilkins, Baltimore, MD, USA (1986)

    Google Scholar 

  20. Shalaby AR. Significance of biogenic amines to food safety and human health. Food Res. Int. 29: 675–690 (1996)

    Article  CAS  Google Scholar 

  21. Hassaïne O, Zadi-Karam H, Karam N-E. Evaluation of biogenic amines formation by proteolytic enterococci strains isolated from raw dromedary milks from southern Algeria. J. Food Safety 29: 381–393 (2009)

    Article  Google Scholar 

  22. Hernández-Herrero MM, Roig-Sagués AX, Rodríguez-Jerez JJ, Mora-Ventura MT. Halotolerant and halophilic histamine-forming bacteria isolated during the ripening of salted anchovies (Engraulis encrasicholus). J. Food Protect. 62: 509–514 (1999)

    Google Scholar 

  23. Mah J-H, Hwang H-J. Effects of food additives on biogenic amine formation in myeolchijeot, a salted and fermented anchovy (Engraulis japonicus). Food Chem. 114: 168–173 (2009)

    Article  CAS  Google Scholar 

  24. Mah J-H, Hwang H-J. Inhibition of biogenic amine formation in a salted and fermented anchovy by Staphylococcus xylosus as a protective culture. Food Control 20: 796–810 (2009)

    Article  CAS  Google Scholar 

  25. Liu Z-F, Wei Y-X, Zhang J-J, Liu D-H, Hu Y-Q, Ye X-Q. Changes in biogenic amines during the conventional production of stinky tofu. Int. J. Food Sci. Tech. 46: 687–694 (2011)

    Article  CAS  Google Scholar 

  26. Kalaè P, Krausová P. A review of dietary polyamines: Formation, implications for growth, and health and occurrence in foods. Food Chem. 90: 219–230 (2005)

    Article  Google Scholar 

  27. Woolridge DP, Vazquez-Laslop N, Markham PN, Chevalier MS, Gerner EW, Neyfakh AA. Efflux of the natural polyamine spermidine facilitated by the Bacillus subtilis multidrug transporter Blt. J. Biol. Chem. 272: 8864–8866 (1997)

    Article  CAS  Google Scholar 

  28. Righetti L, Tassoni A, Bagni N. Polyamines content in plant derived food: A comparison between soybean and Jerusalem artichoke. Food Chem. 111: 852–856 (2008)

    Article  CAS  Google Scholar 

  29. Bills DD, Hildrum KI, Scanlan RA, Libbey LM. Potential precursors of n-nitrosopyrrolidine in bacon and other fried foods. J. Agr. Food Chem. 21: 876–877 (1973)

    Article  CAS  Google Scholar 

  30. Adachi O, Yamada H, Ogata K. Purification and properties of putrescine oxidase of Micrococcus rubens. Agr. Biol. Chem. Tokyo 31: 1202–1210 (1966)

    Article  Google Scholar 

  31. Dasu VV, Nakada Y, Ohnishi-Kameyama M, Kimura K, Itoh Y. Characterization and a role of Pseudomonas aeruginosa spermidine dehydrogenase in polyamine catabolism. Microbiology+ 152: 2265–2272 (2006)

    Article  CAS  Google Scholar 

  32. Padmanabhan R, Tchen TT. Aminoaldehyde dehydrogenases from a Pseudomonas species grown on polyamines. Arch. Biochem. Biophys. 150: 531–541 (1972)

    Article  CAS  Google Scholar 

  33. Fouad KE, Hegeman GD. Microbial spoilage of tofu (soybean curd). J. Food Protect. 56: 157–164 (1993)

    Google Scholar 

  34. Kim B, Byun BY, Mah J-H. Biogenic amine formation and bacterial contribution in natto products. Food Chem. 135: 2005–2011 (2012)

    Article  CAS  Google Scholar 

  35. Byun BY, Mah J-H. Occurrence of biogenic amines in miso, Japanese traditional fermented soybean paste. J. Food Sci. 77: T216–T223 (2012)

    Article  Google Scholar 

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Correspondence to Jae-Hyung Mah.

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Byun, B.Y., Bai, X. & Mah, JH. Occurrence of biogenic amines in Doubanjiang and Tofu . Food Sci Biotechnol 22, 55–62 (2013). https://doi.org/10.1007/s10068-013-0008-x

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