Food Science and Biotechnology

, Volume 21, Issue 5, pp 1309–1315 | Cite as

Survival of probiotic strains in non-dairy indian spice condiment exhibiting cholesterol reducing properties

  • Mukesh Kumar Singh
  • Richu Singla
  • Arashdeep Singh
  • Moushumi Ghosh
  • Abhijit Ganguli
Research Article

Abstract

The feasibility of a novel formulated spice condiment suitable for delivery of probiotic cultures to consumers has been reported in this study. The spice condiment (pH 4.5, Aw=0.81) exhibited high sensorial scores and stability after storage for 60 days. Lactobacillus casei and Lactobacillus delbrueckii strains were incorporated in the spice condiment and evaluated for survival and expression of important functional characteristics: cholesterol reduction. Both strains survived excellently in the food matrix and sustained simulated gastro-intestinal conditions (viability to 0.3% bile salts and pancreatic enzymes). Probiotic enriched spice condiment fed to experimental mice on high cholesterol diet resulted in significant (p<0.05) lowering of serum total cholesterol, HDLs, LDLs, and triglycerides; moreover; histopathological changes were less intensive. Results of our study suggested the formulated spice condiment as a potential non-dairy food adjunct for delivery of probiotics and may help in managing hypercholesterolaemia.

Keywords

spice condiment cholesterol gastric stress lactic acid bacteria probiotic 

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References

  1. 1.
    Verschuren PM. Summary report — Functional foods: Scientific and global perspectives. Brit. J. Nutr. 88: 125–130 (2002)Google Scholar
  2. 2.
    Betoret N, Puente L, Díaz MJ, Pagán MJ, García MJ, Gras ML, Martínez-Monzó J, Fito P. Development of probiotic-enriched dried fruits by vacuum impregnation. J. Food Eng. 56: 273–277 (2003)CrossRefGoogle Scholar
  3. 3.
    Shah NP. Functional cultures and health benefits. Int. Dairy J. 17: 1262–1277 (2007)CrossRefGoogle Scholar
  4. 4.
    Sindhu SC, Khetarpaul N. Effect of probiotic fermentation on antinutrients and in vitro protein and starch digestibilities of indigenously developed RWGT food mixture. Nutr. Health 16: 173–181 (2002)CrossRefGoogle Scholar
  5. 5.
    Leatherhead Food International. The International Market for Functional Foods. 3rd ed. Functional Food Market Report (ISBN-1-904007-82-1). Leatherhead Research Centre, Leatherhead, UK (2006)Google Scholar
  6. 6.
    Sarrela M, Mogensen G, Fonden R, Matto J, Mattila-Sandholm T. Probiotic bacteria: Safety, functional, and technological properties. J. Biotechnol. 84: 197–215 (2000)CrossRefGoogle Scholar
  7. 7.
    Granato D, Branco GF, Nazzaro F, Cruz AG, Faria JAF. Functional foods and nondairy probiotic food development: Trends, concepts, and products. Compr. Rev. Food Sci. F. 9: 292–302 (2010)CrossRefGoogle Scholar
  8. 8.
    Gonza’lez-Fandos ME, Sierra ML, Garcia-Lopez ML, Otero A, Sanz J. Effect of the major herbs and spices in Spanish fermented sausages on Staphylococcus aureus and lactic acid bacteria. Arch. Lebensmittelhyg. 47: 43–47 (1996)Google Scholar
  9. 9.
    Saarela M, Virkajarvi I, Alakomi HL, Mattila PS, Matto J. Stability and functionality of freeze-dried probiotic bifidobacterium cells during storage in juice and milk. Int. Dairy J. 16: 1477–1482 (2006)CrossRefGoogle Scholar
  10. 10.
    Kumar RS, Suresh CG, Brannigan JA, Dodson GG, Gaikwad SM. Bile salt hydrolase, the member of Ntn-hydrolase family: Differential modes of structural and functional transitions during denaturation. IUBMB Life 59: 118–125 (2007)CrossRefGoogle Scholar
  11. 11.
    El-Shafie HA, Yahia NI, Ali HA. Khalil FA, El-Kady EM, Moustafa YA. Hypocholesterolaemic action of Lactobacillus plantarum NRRL-B-4524 and Lactobacillus paracasei in mice with hypercholesterolaemia induced by diet. Aust. J. Basic Appl. Sci. 3: 218–228 (2009)Google Scholar
  12. 12.
    Liong MT, Shah NP. Effects of a Lactobacillus casei symbiotic on serum lipoprotein, intestinal microflora, and organic acids in rats. J. Dairy Sci. 89: 1390–1399 (2006)CrossRefGoogle Scholar
  13. 13.
    Rossi EA, Cavallini DCU, Carlos IZ, Vendramini RC, Damaso AR, de Valdez GF. Intake of isoflavone-supplemented soy yogurt fermented with Enterococcus faecium lowers serum total cholesterol and non-HDL cholesterol of hypercholesterolaemic rats. Eur. Food Res. Technol. 228: 275–282 (2008)CrossRefGoogle Scholar
  14. 14.
    Moretti VM, Madonia G, Diaferia C, Men-Tasti T, Paleari MA, Panseri S, Pirone G, Gandini G. Chemical and micro-biological parameters and sensory attributes of a typical Sicilian salami ripened in different conditions. Meat Sci. 66: 845–854 (2004)CrossRefGoogle Scholar
  15. 15.
    Kumar M, Ghosh M, Ganguli A. Mitogenic response and probiotic characteristics of lactic acid bacteria isolated from indigenously pickled vegetables and fermented beverages. World J. Microbiol. Biot. 28: 703–711 (2012)CrossRefGoogle Scholar
  16. 16.
    Rudel LL, Morris MD. Determination of cholesterol using Ophthalaldehyde. J. Lipid Res. 14: 364–366 (1973)Google Scholar
  17. 17.
    Charteris WP, O’Neill ET, Kelly PM. An in vitro method to determine human gastric transit tolerance among potentially probiotic lactic acid bacteria and bifidobacteria. Ir. J. Agr. Food Res. 33: 203–204 (1994)Google Scholar
  18. 18.
    Xanthopoulus V, Litopoulou-Tzanetaki E, Tzanetakis N. Characterization of Lactobacillus isolates from infant faeces as dietary adjuncts. Food Microbiol. 17: 205–215 (2000)CrossRefGoogle Scholar
  19. 19.
    Hirsch J, Gallian E. Methods for the determination of adipose cell size in man and animals. J. Lipid Res. 9: 110–119 (1968)Google Scholar
  20. 20.
    Motulsky H. Intuitive Biostatistics. Oxford University Press, New York, NY, USA. p. 374 (1995)Google Scholar
  21. 21.
    Sheehan VM, Ross P, Fitzgerald GF. Assessing the acid tolerance and the technological robustness of probiotic cultures for fortification in fruit juices. Innov. Food Sci. Emerg. 8: 279–284 (2007)CrossRefGoogle Scholar
  22. 22.
    Lipid Research Clinics Program. The lipid research clinics coronary primary prevention trial results. 1. Reduction in incidence of coronary heart disease. J. Am. Med. Assoc. 251: 351–363 (1984)CrossRefGoogle Scholar
  23. 23.
    Harrison VC, Peat G. Serum cholesterol and bowel flora in the newborn. Am. J. Clin. Nutr. 28: 1351–1355 (1975)Google Scholar
  24. 24.
    Pulusani SR, Rao DR. Whole body, liver, and plasma cholesterol levels in rats fed Thermophilus, Bulgaricus, and Acidophilus milks. J. Food Sci. 48: 280–281 (1983)CrossRefGoogle Scholar
  25. 25.
    Pereira DI, McCartney LA, Gibson GR. An in vitro study of probiotic potential of a bile salt hydrolyzing Lactobacillus fermentum strain and determination of its cholesterol lowering properties. Appl. Environ. Microb. 69: 4743–4752 (2003)CrossRefGoogle Scholar
  26. 26.
    Dora I, Pereira A, Glenn R, Gibson GR. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Appl. Environ. Microb. 68: 4689–4693 (2002)CrossRefGoogle Scholar
  27. 27.
    Zhou JS, Shu Q, Ruterfurd JK, Prasad J, Gopal KP, Gill SH. Acute oral toxicology and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Chem. Toxicol. 38: 153–161 (2000)CrossRefGoogle Scholar
  28. 28.
    Taranto MP, Seama F, Holdago RPA, Valdez FG. Bile salt hydrolase plays a key role on cholesterol removal by Lactobacillus casei. Biotechnol. Lett. 19: 845–847 (1997)CrossRefGoogle Scholar
  29. 29.
    Manson JE, Tosteson H, Ridker MP, Satterfield S, Hebert P, Oconnor TG. The primary prevention of myocardial infection. New Engl. J. Med. 326: 1406–1416 (1992)CrossRefGoogle Scholar
  30. 30.
    Kawase M, Hashinmoto H, Hosoda M, Morita H, Hosono A. Effect of administration of fermented milk containing whey protein concentrate to rats and healthy men on serum lipids and blood pressure. J. Dairy Sci. 83: 255–263 (2000)CrossRefGoogle Scholar
  31. 31.
    Haberer P, Du Toit M, Dicks TML, Ahrens F, Holzapfel HW. Effect of potentially probiotic lactobacilli on faecal enzyme activity in minipigs on a high-fat, high-cholesterol diet-A preliminary in vivo trial. Int. J. Food Microbiol. 87: 287–291 (2003)CrossRefGoogle Scholar
  32. 32.
    Marteau P, Minekus M, Havenar R, Huis JHJ. Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: Validation and the effect of bile. J. Dairy Sci. 80: 1031–1037 (1997)CrossRefGoogle Scholar
  33. 33.
    Lee YK, Salminen S. The coming age of probiotics. Trends Food Sci. Tech. 6: 241–245 (1995)CrossRefGoogle Scholar
  34. 34.
    Lorca GL, Font de Valdez G. A low-pH-inducible, stationary-phase acid tolerance response in Lactobacillus acidophilus CRL 639. Curr. Microbiol. 42: 21–25 (2001)CrossRefGoogle Scholar
  35. 35.
    van de Guchte M, Serror P, Chervaux C, Smokvina T, Ehrlich S, Maguin E. Stress responses in lactic acid bacteria. Anton. Leeuw. Int. T.G. 82: 187–216 (2002)CrossRefGoogle Scholar
  36. 36.
    Talwalkar A, Kailasapathy K. A review of oxygen toxicity in probiotic yogurts: Influence on the survival of probiotic bacteria and protective techniques. Compr. Rev. Food Sci. F. 3: 117–124 (2004)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Mukesh Kumar Singh
    • 1
  • Richu Singla
    • 1
  • Arashdeep Singh
    • 1
  • Moushumi Ghosh
    • 1
  • Abhijit Ganguli
    • 1
  1. 1.Department of Biotechnology & Environmental SciencesThapar UniversityPatialaIndia

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