Inhibitory effects of antagonistic compounds produced from Lactobacillus brevis MLK27 on adhesion of Listeria monocytogenes KCTC3569 to HT-29 cells
Among lactic acid bacteria (LAB) isolated from mustard leaf kimchi, MLK27 was the most efficient strain in terms of adhesion to HT-29 cells. Competitive, exclusive, and displaceable inhibition of Listeria monocytogenes KCTC 3569 adhesion by LAB was strain-dependent. MLK27 strain displayed the highest adhesion inhibition against L. monocytogenes and produced organic acids, hydrogen peroxide, and bacteriocin. Cell-free culture supernatants (100 μL/mL) and bacteriocin (1,280 AU/mL) of MLK27 strain strongly inhibited viable cell counts of L. monocytogenes adhered to HT-29 cells. However, there was no inhibitory effect of pathogen adhesion by bacteriocin treated with protease and inhibition activity of pathogen adhesion was partially decreased by heat-treated bacteriocin. According to biochemical and phylogenetic analysis, MLK27 strain was identified as Lactobacillus brevis MLK27. In conclusion, our results have shown that L. brevis MLK27 has ability to establish in human gastrointestinal tract and to prevent pathogenic L. monocytogenes infections, which is able to be considered as probiotic strains.
Keywordsadhesion bacteriocin hydrogen peroxide Lactobacillus brevis Listeria monocytogenes
Unable to display preview. Download preview PDF.
- 3.Granato D, Perotti F, Masserey I. Cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of Lactobacillus johnsonii La1 to human enterocyte-like Caco-2 cells. Appl. Environ. Microb. 65: 1071–1077 (1999)Google Scholar
- 8.Coconnier MH, Bernet MF, Chauviere G, Servin AL. Adhering heat-killed human Lactobacillus acidophilus, strain LB, inhibits the process of pathogenicity of diarrhoeagenic bacteria in cultures human intestinal cells. J. Diarrhoeal Dis. Res. 11: 235–242 (1993)Google Scholar
- 10.Bernet MF, Brassart D, Neeser JR, Servin AL. Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions. Appl. Environ. Microb. 59: 4121–4128 (1993)Google Scholar
- 16.Mundt JO. Bergey’s manual of systematic bacteriology. pp. 577–592. In: Lactobacillus. Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds). Williams & Wilkins, Baltimore, MD, USA ( 1986)Google Scholar
- 17.Elliot SN, Buret A, McKnight W, Miller MJS, Wallace JL. Bacteria rapidly colonize and modulate healing of gastric ulcers in rats. Am. J. Physiol. 275: G425–G432 (1998)Google Scholar
- 21.Sherman PM, Johnson-Henry KC, Yeung HP, Ngo PSC, Goulet J, Tompkins TA. Probiotics reduce enterohemorrhagic Escherichia coli O157:H7- and enteropathogenic E. coli O126:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements. Infect. Immun. 73: 5183–5188 (2005)CrossRefGoogle Scholar
- 23.De Vuyst L, Vandamme EJ. Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. J. Gen. Microbiol. 138: 571–578 (1992)Google Scholar
- 24.Podolak PK, Zayas JF, Kastner CL, Fung DYC. Inhibition of Listeria monocytogenes and Escherichia coli O157:H7 on beef by application of organic acids. J. Food Protect. 59: 370–373 (1996)Google Scholar
- 25.Ammor S, Tauveron G, Dufour E, Chevallier I. Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility. 1-Screening and characterization of the antibacterial compounds. Food Control 17: 454–461 (2006)CrossRefGoogle Scholar
- 29.Davidson PM, Hoover DG. Lactic acid bacteria. pp. 127–159. In: Antimcrobial Components from Lactic Acid Bacteria. Salminen S, von Wright A (eds). Marcel Dekker, New York, NY, USA (1993)Google Scholar
- 30.Kang TK, Kim WJ. Characterization of an amylase-sensitive bacteriocin DF01 produced by Lactobacillus brevis DF01 isolated from dongchimi, Korean fermented vegetable. Korean J. Food Sci. An. 30: 795–803 (2010)Google Scholar
- 37.Collins MD, Gibson GR. Probiotics, prebiotics, and symbiotics: Approaches for modulating the microbial ecology of the gut. Am. J. Clin. Nutr. 69: 1052S–1057S (1999)Google Scholar