Food Science and Biotechnology

, Volume 21, Issue 2, pp 565–572 | Cite as

Antioxidative properties of onion peel extracts against lipid oxidation in raw ground pork

  • So-Yeon Shim
  • Yun-Sang Choi
  • Hack-Youn Kim
  • Hyun-Wook Kim
  • Ko-Eun Hwang
  • Dong-Heon Song
  • Mi-Ai Lee
  • Ju-Woon Lee
  • Cheon-Jei KimEmail author
Research Article


Onion peels extracted with various concentrations of ethanol were examined for their extraction yield, total phenolic content, DPPH scavenging activity, and reducing power. Onion peel extracted with 50% ethanol exhibited higher extraction yields, total phenolic content, and DPPH scavenging activity than extracts from distilled water, 70 or 95% ethanol. Thus, the antioxidative efficiency to inhibit lipid oxidation of ascorbic acid (0.05%) and 50% ethanol extract of onion peel (0.05, 0.1, and 0.2%) in raw ground pork was investigated during chilled storage (16 days). The pH of the samples decreased considerably during storage (p<0.05). Addition of ethanol onion peel extract significantly improved the thiobarbituric acid reactive substances and peroxide values in raw ground pork meat (p<0.05). Therefore, onion peel extract by 50% ethanol is a very effective inhibitor of lipid oxidation and has potential as a natural antioxidant in raw ground pork.


onion peel ascorbic acid lipid oxidation natural antioxidant ground pork 


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  1. 1.
    Branen AL. Toxicology and biochemistry of butylated hydroxyanisol and butylated hydroxytoluene. J. Am. Oil. Chem. Soc. 5: 59–63 (1975)CrossRefGoogle Scholar
  2. 2.
    Juntachote J, Berghofer E, Siebenhandl S, Bauer F. The antioxidative properties of Holy basil and Galangal in cooked ground pork. Meat Sci. 72: 446–456 (2006)CrossRefGoogle Scholar
  3. 3.
    Hernández-Hernández E, Ponce-Alquicira E, Jaramillo-Flores ME, Legarreta IG. Antioxidant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Sci. 81: 410–417 (2009)CrossRefGoogle Scholar
  4. 4.
    Mildner-Szkudlarz S, Zawirska-Wojtasiak R, Obuchowski W, Goslinski M. Evaluation of antioxidant activity of green tea extract and its effect on the biscuits lipid fraction oxidative stability. J. Food Sci. 74: S362–S370 (2009)CrossRefGoogle Scholar
  5. 5.
    Maisuthisakul P, Gordon MH. Antioxidant and tyrosinase inhibitory activity of mango seed kernel by product. Food Chem. 117: 332–341 (2009)CrossRefGoogle Scholar
  6. 6.
    Naveena BM, Sen AR, Vaithiyanathan S, Babji Y, Kondaiah N. Comparative efficacy of pomegranate juice, pomegranate rind powder extract and BHT as antioxidants in cooked chicken patties. Meat Sci. 80: 1304–1308 (2008)CrossRefGoogle Scholar
  7. 7.
    Knekt P, Järvinen R, Reunanen A, Maatela J. Flavonoid intake and coronary mortality in Finland: A cohort study. Brit. Med. J. 312: 478–481 (1996)CrossRefGoogle Scholar
  8. 8.
    Prakash D, Singh BN, Upadhyay G. Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chem. 102: 1389–1393 (2007)CrossRefGoogle Scholar
  9. 9.
    Nuutila AM, Puupponen-Pimiä R, Aarni M, Oksman-Caldentey KM. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chem. 81: 485–493 (2003)CrossRefGoogle Scholar
  10. 10.
    Lee JH, Kim DH, Shin HS. Influence of onion (Allium cepa L.) on gemotoxic heterocyclic amine formation and overall mutagenicity in fried hamburger patty. Korean J. Food Sci. Technol. 40: 389–393 (2008)Google Scholar
  11. 11.
    Kim SJ, Kim GH. Quantification of quercetin in different parts of onion and its DPPH radical scavenging and antibacterial activity. Food Sci. Biotechnol. 15: 39–43 (2006)Google Scholar
  12. 12.
    Slinkard K. Singleton VL. Total phenol analysis: Automation and comparison with manual methods. Am. J. Enol. Viticult. 28: 49–55 (1997)Google Scholar
  13. 13.
    Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28: 25–30 (1995)CrossRefGoogle Scholar
  14. 14.
    Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44: 307–315 (1986)CrossRefGoogle Scholar
  15. 15.
    Chae HS, Ahn CN, Yoo YM, Park BY, Ham JS, Kim DH, Lee JM, Choi YI. Effect of the scalding temperature at slaughtering process on meat quality and storage properties of chicken. Korean J. Food Sci. An. 24: 115–120 (2004)Google Scholar
  16. 16.
    Nilsson M, Hansson G, Johansson L. Utveckling av gaskromatogrfisk analysmetod för fettsyror I biolohiska prover (Development of gas choromatography analysis for fatty acids in biological sample). Degree project, University of Göteborg, Sweden (1994)Google Scholar
  17. 17.
    Undeland I, Härröd M, Lingnert H. Comparison between methods using low-toxicity solvents for the extraction of lipids from herring (Clupea harengus). Food Chem. 61: 355–365 (1998)CrossRefGoogle Scholar
  18. 18.
    International IDF Standards. International Dairy Federation. Sec. 74A. IDF-square Vergote 41, Brussels, Belgium (1991)Google Scholar
  19. 19.
    Shantha NC, Decker EA. Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids. J. AOAC. Int. 77: 421–424 (1994)Google Scholar
  20. 20.
    Prasetyo M, Chia M, Hughey C, Were LM. Utilization of electron beam irradiated almond skin powder as a natural antioxidant in ground top round beef. J. Food Sci. 73: T1–T6 (2008)CrossRefGoogle Scholar
  21. 21.
    SAS Institute, Inc. SAS User’s Guide. Statistical Analysis Systems Institute, Cary, NC, USA (2001)Google Scholar
  22. 22.
    Lee MA, Choi JH, Choi YS, Kim HY, Kim HW, Hwang KE, Chung HK, Kim CJ. Effect of kimchi ethanolic extracts on oxidative stability of refrigerated cooked pork. Meat Sci. 89: 405–411 (2011)CrossRefGoogle Scholar
  23. 23.
    Ra KS, Suh HJ, Chung SH, Son JY. Antioxidant activity of solvent extract from onion skin. Korean J. Food Sci. Technol. 29: 595–600 (1997)Google Scholar
  24. 24.
    Choe JH, Jang A, Lee ES, Choi JH, Choi YS, Han DJ, Kim HY, Lee MA, Shim SY, Kim CJ. Oxidative and color stability of cooked ground pork containing lotus leaf (Nelumbo nucifera) and barley leaf (Hordeum vulgare) powder during refrigerated storage. Meat Sci. 87: 12–18 (2011)CrossRefGoogle Scholar
  25. 25.
    Lee MA, Choi JH, Choi YS, Han DJ, Kim HY, Shim SY, Chung HK, Kim CJ. The antioxidative properties of mustard leaf (Brassica juncea) kimchi extracts on refrigerated raw ground pork meat against lipid oxidation. Meat Sci. 84: 498–504 (2010)CrossRefGoogle Scholar
  26. 26.
    Jeong CH, Heo HJ, Choi SG, Shim KH. Antioxidant and anticancer properties of methanolic extracts from different parts of white, yellow, and red onion. Food Sci. Biotechnol. 18: 108–112 (2009)Google Scholar
  27. 27.
    Wang T, Jónsdóttir R, Ólafsdóttir G. Total phenolic compounds, radical scavenging, and metal chelation of extracts from Icelandic seaweeds. Food Chem. 116: 240–248 (2009)CrossRefGoogle Scholar
  28. 28.
    Chang LW, Yen WJ, Huang SC, Duh PD. Antioxidant activity of sesame coat. Food Chem. 78: 347–354 (2002)CrossRefGoogle Scholar
  29. 29.
    Jeong HJ, Park SB, Kim S, Kim HK. Total polyphenol content and antioxidative activity of wild grape (Vitis coignetiae) extracts depending on ethanol concentrations. J. Korean Soc. Food Sci. Nutr. 36: 1491–1496 (2007)CrossRefGoogle Scholar
  30. 30.
    Gordon MF. The mechanism of antioxidant action in vitro. pp. 1–18. In: Food Antioxidants. Hudson BRJ (ed). Elsevier Applied Science, London, UK (1990)CrossRefGoogle Scholar
  31. 31.
    Pearson AM, Young RB. Muscle and Meat Biochemistry. Academic Press, San Diego, CA, USA. pp. 1–261 (1989)Google Scholar
  32. 32.
    Choi YS, Choi JH, Kim HY, Kim HW, Lee MA, Chung HJ, Lee SK, Kim CJ. Effect of lotus (Nelumbo nucifera) leaf powder on the quality characteristics of chicken patties in refrigerated storage. J. Korean Food Sci. An. 31: 9–18 (2011)CrossRefGoogle Scholar
  33. 33.
    Ahn DU, Nam KC. Effects of ascorbic acid and antioxidants on color, lipid oxidation, and volatiles of irradiated ground beef. Radiat. Phys. Chem. 71: 149–154 (2004)Google Scholar
  34. 34.
    Igene JO, Yamauchi K, Pearson AM, Gray JI, Aust SD. Evaluation of 2-thiobarbituric acid reactive substances (TBARS) in relation to warmed-over flavor (WOF) development in cooked chicken. J. Agr. Food Chem. 33: 364–367 (1985)CrossRefGoogle Scholar
  35. 35.
    Hassan O, Fan SF. The anti-oxidation potential of polyphenol extract from cocoa leaces on mechanically deboned chicken meat (MDCM). LWT -Food Sci. Technol. 38: 315–321 (2005)CrossRefGoogle Scholar
  36. 36.
    Lund MN, Hviid MS, Skibsted LH. The combined effect of antioxidants and modified atmosphere packaging on protein and lipid oxidation in beef patties during chill storage. Meat Sci. 76: 226–233 (2007)CrossRefGoogle Scholar
  37. 37.
    Sánchez-Escalante A, Djenane D, Torrescano G, Beltrán JA, Roncales P. Antioxidant action of borage, rosemary, oregano, and ascorbic acid in beef patties packaged in modified atmosphere. J. Food Sci. 68: 339–344 (2003)CrossRefGoogle Scholar
  38. 38.
    Cheng, JH, Wang ST, Ockerman HW. Lipid oxidation and color change of salted pork patties. Meat Sci. 75: 71–77 (2007)CrossRefGoogle Scholar
  39. 39.
    Teets AS, Were LM. Inhibition of lipid oxidation in refrigerated and frozen salted raw minced chicken breasts with electron beam irradiated almond skin powder. Meat Sci. 80: 1326–1332 (2008)CrossRefGoogle Scholar
  40. 40.
    Kulas E, Ackman RG. Different tocopherols and the relationship between two methods for determination of primary oxidation products in fish oil. J. Agr. Food Chem. 49: 1724–1729 (2001)CrossRefGoogle Scholar
  41. 41.
    Sánchez-Escalante A, Djenane D, Torrescano G, Beltrán JA, Roncalés P. The effects of ascorbic acid, taurine, carnosine, and rosemary powder on color and lipid stability of beef patties packaged in modified atmosphere. Meat Sci. 58: 421–429 (2001)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2012

Authors and Affiliations

  • So-Yeon Shim
    • 1
  • Yun-Sang Choi
    • 2
  • Hack-Youn Kim
    • 3
  • Hyun-Wook Kim
    • 1
  • Ko-Eun Hwang
    • 1
  • Dong-Heon Song
    • 1
  • Mi-Ai Lee
    • 4
  • Ju-Woon Lee
    • 5
  • Cheon-Jei Kim
    • 1
    Email author
  1. 1.Department of Food Science and Biotechnology of Animal ResourcesKonkuk UniversitySeoulKorea
  2. 2.Food and Biological Resources Examination DivisionKorean Intellectural Property OfficeDaejeonKorea
  3. 3.Department of Animal Science, College of Industrial ScienceResearch Kongju National UniversityYesan, ChungnamKorea
  4. 4.World Institute of KimchiAn Annex of Korea Food Research InstituteSeongnam, GyeonggiKorea
  5. 5.Radition Technology InstituteKorea Atomic Energy Research InstituteJeongeup, JeonbukKorea

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