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Food Science and Biotechnology

, Volume 21, Issue 1, pp 101–106 | Cite as

Rapid detection of food-borne Listeria monocytogenes by real-time quantitative loop-mediated isothermal amplification

  • Xiaoxiao Shan
  • Yaoqi Zhang
  • Zhigang Zhang
  • Miaorui Chen
  • Yongyu Su
  • Yingna Yuan
  • M. Jahangir Alam
  • He Yan
  • Lei ShiEmail author
Research Article

Abstract

The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at 63°C with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6 colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional methodpositive samples was missed by the LAMP method.

Keywords

Listeria monocytogenes loop-mediated isothermal amplication (LAMP) real-time turbidimeter quantitative 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2012

Authors and Affiliations

  • Xiaoxiao Shan
    • 2
    • 3
  • Yaoqi Zhang
    • 1
    • 3
  • Zhigang Zhang
    • 3
  • Miaorui Chen
    • 3
  • Yongyu Su
    • 3
  • Yingna Yuan
    • 4
  • M. Jahangir Alam
    • 5
  • He Yan
    • 1
    • 3
  • Lei Shi
    • 1
    • 3
    Email author
  1. 1.College of Light Industry and Food SciencesSouth China University of TechnologyGuangzhouChina
  2. 2.School of Bioscience and BioengineeringSouth China University of TechnologyGuangzhouChina
  3. 3.State Key Laboratory of Food Safety Technology for Meat ProductsXiamen Yinxiang Group Co., Ltd.XiamenChina
  4. 4.College of Landscape and ArtJiangxi Agricultural UniversityNanchang, JiangxiChina
  5. 5.Texas Commission on Environmental QualityHoustonUSA

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