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Food Science and Biotechnology

, 20:1457 | Cite as

Rapid concentration of some bacteriocin-like compounds using an organic solvent

  • Dong-Min Chung
  • Ki Eun Kim
  • Seong-Yeop Jeong
  • Chang-Su Park
  • Keug-Hyun Ahn
  • Dong Ho Kim
  • Dae-Ook Kang
  • Hyo Kon Chun
  • Byung-Dae Yoon
  • Hong Bum Koh
  • Hong Jib Kim
  • Nack-Shick ChoiEmail author
Research Note

Abstract

A new solvent extraction method for isolation of bacteriocin-like compounds (BLCs) from bacterial culture broth was developed. Culture supernatant of Pediococcus acidilactici WRL-1 was mixed with 3 volumes of cold acetone and maintained at −20°C for 2 h. The solvent and precipitate were separated by centrifugation. Most (95.2 ±2.5%) pediocin activity was found in the liquid phase and it was obtained by evaporation. This method was also an effective means of isolating other BLCs from cultures of Lactobacillus acidophilus, Lactobacillus plantarum, and Bacillus licheniformis DJ-2. Other solvents (methanol, ethanol, and butanol) were also suitable toward this end.

Keywords

bacteriocin-like compound organic solvent anti-microbial Bacillus cereus 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2011

Authors and Affiliations

  • Dong-Min Chung
    • 2
  • Ki Eun Kim
    • 1
    • 6
  • Seong-Yeop Jeong
    • 2
    • 5
  • Chang-Su Park
    • 3
  • Keug-Hyun Ahn
    • 2
  • Dong Ho Kim
    • 4
  • Dae-Ook Kang
    • 5
  • Hyo Kon Chun
    • 2
  • Byung-Dae Yoon
    • 2
  • Hong Bum Koh
    • 6
  • Hong Jib Kim
    • 1
  • Nack-Shick Choi
    • 1
    Email author
  1. 1.Woori Life Science Co., Ltd.Cheonan, ChungnamKorea
  2. 2.Institute Bioindustry Research CenterKRIBBJeongeup, JeonbukKorea
  3. 3.Department of Food Science & TechnologyCatholic University of DaeguGyeongsan, GyeongbukKorea
  4. 4.Advanced Radiation Technology Research InstituteKAERI, Jeonbuk Branch InstituteJeongeup, JeonbukKorea
  5. 5.Department of Biochemistry and Health ScienceChangwon National UniversityChangwon, GyeongnamKorea
  6. 6.Colleage of Veterinary MedicineChonnam National UniversityGwangjuKorea

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