Food Science and Biotechnology

, Volume 20, Issue 2, pp 311–318 | Cite as

Neuronal cell protection and acetylcholinesterase inhibitory effect of the phenolics in chestnut inner skin

  • Ji Hye Kim
  • Gwi Nam Choi
  • Ji Hyun Kwak
  • Hee Rok Jeong
  • Chang-Ho Jeong
  • Ho Jin Heo
Research Article


Chestnut inner skin was known to contain various phenolics as a potential antioxidant, and 3 phenolics (catechin, epicatechin, and gallic acid) were isolated. The relative antioxidant capacity of 3 phenolics in vitamin C equivalent antioxidant capacity (VCEAC) evaluated by ABTS assay was in decreasing order as follows: gallic acid>catechin>epicatechin>vitamin C. Gallic acid showed the highest inhibitory effect against acetylcholinesterase (AChE) in a dose-dependent manner and competitive inhibition. However catechins presented neuroprotective effects on amyloid β-induced neurotoxicity by MTT, lactate dehydrogenase (LDH) release, and neutral red uptake assays. Considering above data, it was supposed that gallic acid, catechin, and epicatechin have various antiamnesic effect in neurodegenerative diseases such as Alzheimer’s disease (AD). Therefore, it was strongly suggested that chestnut inner skin as phenolics-rich substance can be used in functional food supplement or pharmaceutical industry by utilization of wastage from valuable resources.


acetylcholinesterase (AChE) amyloid β protein catechin epicatechin gallic acid 


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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2011

Authors and Affiliations

  • Ji Hye Kim
    • 1
    • 2
  • Gwi Nam Choi
    • 1
  • Ji Hyun Kwak
    • 1
  • Hee Rok Jeong
    • 1
  • Chang-Ho Jeong
    • 3
  • Ho Jin Heo
    • 1
  1. 1.Department of Food Science and Technology, Institute of Agriculture and Life ScienceGyeongsang National UniversityJinju, GyeongnamKorea
  2. 2.Division of Special-purpose TreesKorea Forest Research InstituteSuwon, GyeonggiKorea
  3. 3.Department of Food Science and Technology, Institute of Life Science and ResourcesKyung Hee UniversityYongin, GyeonggiKorea

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