Food Science and Biotechnology

, Volume 20, Issue 1, pp 151–158

Catechin-7-O-β-d-glucopyranoside scavenges free radicals and protects human B lymphoma BJAB cells on H2O2-mediated oxidative stress

  • Jin-A Baek
  • Young-Ok Son
  • Minghao Fang
  • Young Jae Lee
  • Hyoung-Kwon Cho
  • Wan Kyunn Whang
  • Jeong-Chae Lee
Research Article

DOI: 10.1007/s10068-011-0021-x

Cite this article as:
Baek, JA., Son, YO., Fang, M. et al. Food Sci Biotechnol (2011) 20: 151. doi:10.1007/s10068-011-0021-x

Abstract

Catechin-7-O-β-d-glucopyranoside (CA-G) was previously isolated from red bean (the seed of Phaseolus calcaratus cv. Roxburgh). This study examined the ability of CA-G to scavenge reactive oxygen species generated by cell-free systems and to protect cells from oxidative stress caused by hydrogen peroxide (H2O2). The mechanism by which CA-G exerts its antioxidant and anti-apoptotic action on H2O2-exposed cells was also investigated. CA-G treatment prevented H2O2-mediated apoptosis and inhibited the formation of single stand breaks in DNA in H2O2-exposed BJAB cells. CA-G suppressed mitochondrial stress and caspase activation caused by H2O2. Mechanistic experiments revealed that the antioxidant mechanism of CA-G on H2O2-mediated oxidative damage was due to the direct scavenging of hydroxyl radicals and/or to the chelation of metal ions that were used to produce hydroxyl radicals from H2O2 via the Fenton reaction. Collectively, these findings suggest beneficial roles of CA-G or CA-G-rich red bean on the protection from oxidative damage.

Keywords

apoptosis catechin-7-O-β-d-glucopyranoside DNA damage hydrogen peroxide oxidative stress red bean 

Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2011

Authors and Affiliations

  • Jin-A Baek
    • 1
  • Young-Ok Son
    • 2
  • Minghao Fang
    • 1
  • Young Jae Lee
    • 3
    • 4
  • Hyoung-Kwon Cho
    • 3
  • Wan Kyunn Whang
    • 4
  • Jeong-Chae Lee
    • 1
    • 5
  1. 1.Institute of Oral Biosciences and BK21 ProgramChonbuk National UniversityJeonju, JeonbukKorea
  2. 2.Graduate Center for Toxicology, College of MedicineUniversity of KentuckyLexingtonUSA
  3. 3.Center for Health Care Technology DevelopmentHanPoong Pharmaceutical Co., Ltd.Jeonju, JeonbukKorea
  4. 4.College of PharmacyChung-Ang UniversitySeoulKorea
  5. 5.Research Center of Bioactive Materials, Institute of Oral Biosciences and BK21 ProgramChonbuk National UniversityJeonju, JeonbukKorea

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