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Application of high-speed counter-current chromatography for the isolation of 5 alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves

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Abstract

A high-speed counter-current chromatography (HSCCC) method was developed for isolation of 5 alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves. The 2-phase solvent system of HSCCC composed of light petroleum-ethyl acetate-methanol-water was set up in 2- step separation process in the proportion of 3:5:3:5 for isolation of N-nornuciferine and armepavine, and in the proportion of 1:5:1:5 for that of anonaine, pronuciferine, and nuciferine. The purity of anonaine (14.6mg), pronuciferine (29.7 mg), N-nornuciferine (31.4 mg), nuciferine (22.1 mg), and armepavine (23.3 mg) isolated from 150 mg crude extract of lotus leaves were examined as 95.6, 88.2, 92.5, 94.3, and 92.1%, respectively.

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Authors and Affiliations

  1. State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi, 330047, China

    Jiang-Ning Hu, Bin Shan, Ze-Yuan Deng, Jing Li, Ya-Wei Fan, Zheng Ruan & Rong Liu

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  1. Jiang-Ning Hu
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  2. Bin Shan
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  3. Ze-Yuan Deng
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  4. Jing Li
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  5. Ya-Wei Fan
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  6. Zheng Ruan
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  7. Rong Liu
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Correspondence to Ze-Yuan Deng.

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Hu, JN., Shan, B., Deng, ZY. et al. Application of high-speed counter-current chromatography for the isolation of 5 alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves. Food Sci Biotechnol 19, 1661–1665 (2010). https://doi.org/10.1007/s10068-010-0235-3

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  • DOI: https://doi.org/10.1007/s10068-010-0235-3

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