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Food Science and Biotechnology

, Volume 19, Issue 5, pp 1325–1332 | Cite as

α-pinene triggers oxidative stress and related signaling pathways in A549 and HepG2 cells

  • Kyong-Suk Jin
  • Min-Ji Bak
  • Mira Jun
  • Ho-Jin Lim
  • Wan-Keun Jo
  • Woo-Sik JeongEmail author
Research Article

Abstract

The volatile organic compounds (VOCs) -dlimonene, α-pinene, and isoprene-are widely used in scented products including food flavorings, air fresheners, and cosmetics. The effects of these VOCs on cell viability, oxidative stress generation, and the related molecular events were investigated in A549 and HepG2 cells. α-Pinene induced cell death, reactive oxygen species (ROS) generation, and reporter gene activities of antioxidant response element (ARE), activator protein 1 (AP-1), and nuclear factor (NF)-κB. α-Pinene stimulated the redoxsensitive transcription factors such as nuclear factor E2-related factor 2 (Nrf2) and NF-κB. Their down stream targets including heme oxygenase 1, inducible nitric oxide synthase, and cyclooxygenase 2 also increased after treatment with α-pinene. Moreover, α-pinene-induced oxidative stress-related signaling pathways could be connected by mitogen-activated protein kinases. Conversely, α-pinene-mediated cell death, ROS formation, and AP-1 induction were inhibited by an antioxidant, N-acetyl-l-cysteine. Thus, α-pinene can induce the cell death possibly by modulating oxidative stress-related signaling pathways which can be reversed by an antioxidant treatment.

Keywords

volatile organic compound α-pinene oxidative stress cytotoxicity activator protein 1 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2010

Authors and Affiliations

  • Kyong-Suk Jin
    • 1
  • Min-Ji Bak
    • 1
  • Mira Jun
    • 2
  • Ho-Jin Lim
    • 3
  • Wan-Keun Jo
    • 3
  • Woo-Sik Jeong
    • 1
    Email author
  1. 1.School of Food and Life Sciences, College of Biomedical Science and Engineering, Center of Smart Foods & DrugsInje UniversityGimhae, GyeongnamKorea
  2. 2.Division of Food ScienceDong-A UniversityBusanKorea
  3. 3.Department of Environmental EngineeringKyungpook National UniversityDaeguKorea

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