Selection of psychrotrophic Leuconostoc spp. from native fruits, and studies on their dextransucrases
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Present study was aimed at selection of psychrotrophic Leuconostoc spp., having dextransucrase activity from native fruits and sauerkraut. Fifty-six colonies of lactic acid bacteria producing dextransucrase were isolated and characterized by physiological-biochemical methods. Seven isolates with high dextransucrase activities (0.68–1.63 IU/mL) were studied in detail including characterization by 16S rDNA sequence comparison. It was found that these isolates grow faster and produced dextransucrase earlier than Leuconostoc mesenteroides B512FMC-13Glc, a mutant form of the commercial strain. Molecular weights of the dextransucrases produced by 7 isolates were determined and acceptor reactions using maltose were studied. Dextranase hydrolysis products of dextrans produced by new enzymes were similar to those of commercially produced B-512 F dextran. The new dextransucrases could find industrial uses and the strains isolated (AN 39-1, Or2p-2, Mu3, and Mu10) could be used as prebiotic producing starters in food fermentations due to having high dextransucrase activity and their growth at 4°C.
Keywordsacceptor reaction dextransucrase fruit Leuconostoc mesenteroides psychrotroph
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