Food Science and Biotechnology

, Volume 19, Issue 1, pp 27–33 | Cite as

Characterization of acid- and pepsin-soluble collagens from flatfish skin

  • Min Soo Heu
  • Jae Hyoung Lee
  • Hyung Jun Kim
  • Seong Joon Jee
  • Jung Suck Lee
  • You-Jin Jeon
  • Fereidoon Shahidi
  • Jin-Soo Kim
Research Article
First Online:
Received:
Revised:
Accepted:

Abstract

Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from flatfish skin were characterized. The yield of PSC was 85.5%, which was higher than that of ASC at 57.3%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns showed that both the ASC and PSC were consisted of α1 and α2 chains, and β-chain. According to the results of Fourier transform infrared (FTIR) spectroscopy and hydroxylation, no difference in the helical structure between ASC and PSC was found. Thermal denaturation temperature (TDT) of flatfish skin collagen was 26.6°C for ASC and 26.7°C for PSC, which were similar to those of temperate fishes, while were lower compared to tropical fishes, such as the conger eel and Nile perch. There was no solubility difference between ASC and PSC at various pH and NaCl concentrations. Therefore, PSC will be preferable compared to ASC for commercial uses because of its higher yield.

Keywords

collagen fish collagen flatfish skin flatfish 
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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2010

Authors and Affiliations

  • Min Soo Heu
    • 2
  • Jae Hyoung Lee
    • 1
  • Hyung Jun Kim
    • 1
  • Seong Joon Jee
    • 1
  • Jung Suck Lee
    • 3
  • You-Jin Jeon
    • 4
  • Fereidoon Shahidi
    • 5
  • Jin-Soo Kim
    • 1
  1. 1.Department of Seafood Science and Technology/Institute of Marine IndustryGyeongsang National UniversityTongyeong, GyeongnamKorea
  2. 2.Department of Food Science and Nutrition /Institute of Marine IndustryGyeongsang National UniversityJinju, GyeongnamKorea
  3. 3.Aqua Green Technology Co., Ltd.Jeju, JejuKorea
  4. 4.Department of Marine BiotechnologyCheju National UniversityJeju, JejuKorea
  5. 5.Department of BiochemistryMemorial University of NewfoundlandSt. John’sCanada

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