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Food Science and Biotechnology

, Volume 19, Issue 1, pp 19–25 | Cite as

In vitro evaluation of probiotic lactic acid bacteria isolated from dairy and non-dairy environments

  • Tae-Suk Song
  • Ji-Youn Kim
  • Ki-Hwan Kim
  • Byung-Moon Jung
  • Soong-Seop Yun
  • Sung-Sik YoonEmail author
Research Article

Abstract

Four strains and 2 strains of lactic acid bacteria (LAB) were isolated from the commercial yogurt and kimchi products in Korea, respectively. Based on the 16S rRNA sequencing data, strain A from a drink-type yogurt manufactured by dairy company S, was a Gram-positive, rod-shaped Lactobacillus helveticus, and both strain B (company N) and D (company H) were identified as L. casei ssp. casei, and strain C (company L) as L. paracasei. None of yogurt strain B and D was recovered from the samples exposed to the simulated gastric juice, pH 2.0 for 1.5 hr. Of the 6 isolates tested, strain YS93 from kimchi was the most resistant to acidic condition using the simulated gastric juice, pH 2.0. Moreover, it was shown that 2 kimchi isolates and yogurt strain D produced antibacterial substances, probably bacteriocin-like peptide, which was inhibitory against Staphylococcus aureus as an indicator. In an adhesion assay using a Caco-2 cell, the adherence activity of kimchi strains YS29 and YS93 was significantly higher than those of 4 yogurt starter strains tested.

Keywords

lactic acid bacteria probiotic identification adherence Caco-2 cell 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2010

Authors and Affiliations

  • Tae-Suk Song
    • 1
  • Ji-Youn Kim
    • 1
  • Ki-Hwan Kim
    • 1
  • Byung-Moon Jung
    • 2
  • Soong-Seop Yun
    • 2
  • Sung-Sik Yoon
    • 1
    Email author
  1. 1.Department of Life Science and Technology, College of Science and EngineeringYonsei UniversityWonju, GangwonKorea
  2. 2.RsD Center Maeil Dairies Co., Ltd.Pyungtaek, GyeonggiKorea

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