Abstract
Objective
Rheumatoid arthritis (RA) is a chronic inflammatory and angiogenic disease. This study aimed to explore the profiles of circulating angiogenic T cells (Tang cells) and the role of CD147 in Tang cell activation function in RA.
Methods
Samples were obtained from patients with RA and health controls (HC). Then, Tang cells were quantified by flow cytometry (FCM) in the samples from 87 patients with RA and 29 HC. Tang cells were purified by magnetic cell sorting in cell culture–conditioned media, and the phosphorylation signals were determined by FCM. In addition, cytokine levels were assessed by enzyme-linked immunosorbent assay.
Results
The percentage of circulating Tang cells increased and positively correlated with the number of endothelial progenitor cells in the RA group. Further, the percentage of Tang cells was closely related to disease activity, autoantibody positivity, and proangiogenic cytokine levels. Meanwhile, the expression of CD147 on Tang cells increased in patients with RA. CD147 participated in the Akt phosphorylation and VEGF level of the activated Tang cells.
Conclusions
CD147 may play a critical role in regulating VEGF production of activated Tang cells by affecting Akt signaling, which in turn may serve an essential function in angiogenesis and RA pathogenesis.
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Funding
This work was supported by grants from the National Key Research and Development Program of China (no. 2017YFC0909000) and the National Natural Science Foundation of China (no. 81871273 and no.81801599).
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Our research was approved by ethical standards committee of Xijing Hospital, Fourth Military Medical University, and all the participants gave written informed consent (REB registration number: XJYYLL-2014142).
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Zhao, P., Miao, J., Zhang, K. et al. CD147 participates in the activation function of circulating angiogenic T cells in patients with rheumatoid arthritis. Clin Rheumatol 38, 2621–2628 (2019). https://doi.org/10.1007/s10067-019-04584-4
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DOI: https://doi.org/10.1007/s10067-019-04584-4