Clinical Rheumatology

, Volume 38, Issue 1, pp 117–124 | Cite as

Vitamin D receptor gene polymorphism influences lipid profile in patients with juvenile idiopathic arthritis

  • Jelena BašićEmail author
  • Jelena Vojinović
  • Tatjana Jevtović-Stoimenov
  • Milena Despotović
  • Gordana Sušić
  • Dragana Lazarević
  • Vuk Milošević
  • Mina Cvetković
  • Dušica Pavlović
Original Article


Vitamin D receptor (VDR) gene FokI (rs2228570) polymorphism was postulated to influence outcome of several inflammatory diseases. The aim of this study was to evaluate the influence of rs2228570 polymorphism on lipid profile and on outcome in patients with juvenile idiopathic arthritis (JIA) treated with etanercept. A total of 153 subjects (62 JIA patients and 91 controls) were screened for the rs2228570 using the PCR-RFLP method. Lipid profile (cholesterol, triacylglycerol, HDL-C, and LDL-C) was determined using standard biochemical analysis in controls, while in JIA patients, it was determined prior to and 12 months after anti-TNF (etanercept) therapy. Clinical outcome was assessed using the JIA—American College of Rheumatology (ACR) response criteria. There were significant differences in the distribution of genotypes (p = 0.024) and alleles (p = 0.006; OR = 2.222, 95% CI 1.136–4.348) of the rs2228570 between patients and controls. Etanercept treatment significantly increased HDL-C levels (p = 0.006) in JIA patients with FF genotype in comparison to baseline values. No significant differences were seen in JIA—ACR 30/50/70 responses at month 12 between FF and Ff/ff genotype carriers. This is the first study to demonstrate the protective effect of the VDR FokI FF genotype on lipid profile in JIA patients treated with etanercept. However, this has to be confirmed in a larger cohort of patients.


Etanercept FokI polymorphism Juvenile idiopathic arthritis Lipid profile 


Funding information

This work was supported by the Ministry of Education, Science, and Technological Development of the Republic of Serbia (grant number III41018).

Compliance with ethical standards

All participants voluntarily agreed to participate in the research, and informed consent was signed by parents or by the patients if they were aged ≥ 12 years. The Ethical Committee of the Medical Faculty in Niš gave consent for conducting this study. All participants were treated in accordance with the Helsinki Declaration. The research was conducted in the Laboratory for Functional Genomics and Proteomics, at the Medical Faculty, University of Niš, Serbia.

Conflict of interests

The authors declare that they have no conflict of interest.


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Copyright information

© International League of Associations for Rheumatology (ILAR) 2018

Authors and Affiliations

  • Jelena Bašić
    • 1
    Email author
  • Jelena Vojinović
    • 2
    • 3
  • Tatjana Jevtović-Stoimenov
    • 1
  • Milena Despotović
    • 1
  • Gordana Sušić
    • 4
  • Dragana Lazarević
    • 2
  • Vuk Milošević
    • 5
  • Mina Cvetković
    • 3
  • Dušica Pavlović
    • 1
  1. 1.Faculty of Medicine, Department of BiochemistryUniversity of NišNišSerbia
  2. 2.Clinic of PediatricsClinical Center NišNišSerbia
  3. 3.Faculty of MedicineUniversity of NišNišSerbia
  4. 4.Institute of RheumatologyBelgradeSerbia
  5. 5.Clinic of NeurologyClinical Center NišNišSerbia

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