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Relative abundance of Megamonas hypermegale and Butyrivibrio species decreased in the intestine and its possible association with the T cell aberration by metabolite alteration in patients with Behcet’s disease (210 characters)

  • Jun Shimizu
  • Takao Kubota
  • Erika Takada
  • Kenji Takai
  • Naruyoshi Fujiwara
  • Nagisa Arimitsu
  • Yuji Ueda
  • Sueshige Wakisaka
  • Tomoko Suzuki
  • Noboru SuzukiEmail author
Original Article

Abstract

Objectives

We have previously demonstrated that the phylum Actinobacteria, the family Lactobacillaceae, and the genus Bifidobacterium increased in relative abundance of gut microbiota in patients with Behcet’s disease (BD). The phylum Firmicutes and the class Clostridia were predominant in the feces of normal individuals. The class Clostridia includes short-chain fatty acid–producing bacteria, important for the balance between regulatory T cells and helper T type 17 (Th17) cells. It is possible that the bacterial compositional alteration causes low intestinal short-chain fatty acid concentrations, leading to skewed immune functions in patients with BD.

Methods

To test the hypothesis, we examined species composition and gene functions from the 16S rRNA data by utilizing PICRUSt software.

Results

We have shown that relative abundance of Eggerthella lenta, Acidaminococcus species, Lactobacillus mucosae, Bifidobacterium bifidum, Lactobacillus iners, Streptococcus species, and Lactobacillus salivarius increased significantly in patients with BD. Relative abundance of Megamonas hypermegale, Butyrivibrio species, Streptococcus infantis, and Filifactor species increased significantly in normal individuals compared with BD patients. In the functional annotation analysis by PICRUSt, we found prevalent gene functions of the pentose phosphate pathway and the inosine monophosphate biosynthesis in patients with BD. The data suggested that BD gut microbes altered nucleic acid and fatty acid synthesis.

Conclusions

These compositional and functional alterations of gut microbes may accompany unfavorable molecular exchanges between intestinal immunocompetent cells and gut microbes, and these interactions may have an association with the immune aberration in patients with BD.

Keywords

Behcet’s disease Butyrivibrio species Inosine monophosphate biosynthesis Megamonas hypermegale Pentose phosphate pathway Th17 cells 

Notes

Acknowledgements

The authors are grateful to Dr. Masanori A. Murayama (Department of Immunology and Medicine, St. Marianna University School of Medicine) for his help in the review process.

Funding

This study was supported in part by Grants-in-Aid for Scientific Research (Grant number: 17K10009) of Japan Society for the Promotion of Science.

Compliance with ethical standards

This study was approved by the institutional review boards of St. Marianna University School of Medicine and was registered with the University Hospital Medical Information Network-Clinical Trials Registry (UMIN000018937). We conducted this research according to the principles expressed in the Declaration of Helsinki. We obtained written informed consent from each individual prior to enrolment in the study. A copy of the written consent is available for review upon request.

Conflict of interest

The authors have no conflict of interest.

Supplementary material

10067_2018_4419_MOESM1_ESM.pdf (386 kb)
ESM 1 (PDF 386 kb)

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Copyright information

© International League of Associations for Rheumatology (ILAR) 2019

Authors and Affiliations

  • Jun Shimizu
    • 1
  • Takao Kubota
    • 1
  • Erika Takada
    • 1
  • Kenji Takai
    • 1
  • Naruyoshi Fujiwara
    • 1
  • Nagisa Arimitsu
    • 1
  • Yuji Ueda
    • 1
  • Sueshige Wakisaka
    • 1
  • Tomoko Suzuki
    • 1
  • Noboru Suzuki
    • 1
    Email author
  1. 1.Department of Immunology and MedicineSt. Marianna University School of MedicineMiyamae-kuJapan

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