Culture, PCR, DNA sequencing, and second harmonic generation (SHG) visualization of Bartonella henselae from a surgically excised human femoral head
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Bartonella henselae is an emerging, zoonotic pathogen that appears to represent an occupational risk for individuals with arthropod exposures and animal contact [1, 2]. Rheumatic and musculoskeletal abnormalities have been previously reported in patients with or following historical cat scratch disease (CSD), caused predominantly or solely by B. henselae [3, 4, 5] In contrast to the classical self-limiting clinical presentation of fever and lymphadenopathy and a history of cat scratch in patients with CSD, recent evidence supports the possibility of persistent intravascular infections in previously immunocompetent patients with accompanying rheumatic symptoms [1, 6]. Historically, systemic bartonellosis has been reported in immunocompromised patients, such as HIV/AIDS or transplant recipients . Recently, Bartonellaspp. bacteremia has been reported in healthy Brazilian blood donors and in previously healthy, immunocompetent patients who developed chronic and often...
KeywordsFemoral Head Second Harmonic Generation Spongy Tissue Second Harmonic Generation Signal Bartonella Henselae
The authors thank Keith T. Brickman for facilitating rapid transport of the femoral head from the surgery site to the NCSU-CVM IPRL for microbiological culture. This research was supported in part by unrestricted donations to the NCSU-CVM Foundation for Vector Borne Diseases Research.
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Conflict of interest
In conjunction with Dr. Sushama Sontakke and North Carolina State University, Edward B. Breitschwerdt, DVM, holds US Patent No. 7,115,385; Media and methods for cultivation of microorganisms, which was issued on October 3, 2006. He is a co-founder, stock holder, and chief scientific officer for Galaxy Diagnostics, a company that provides advanced diagnostic testing for the detection of Bartonella species infections. Robert B. Mozayeni, MD, is chief medical officer for Galaxy Diagnostics. The remaining authors have no competing interests.
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