Clinical Rheumatology

, Volume 31, Issue 6, pp 951–959 | Cite as

Changes in immune cell frequencies after cyclophosphamide or mycophenolate mofetil treatments in patients with systemic lupus erythematosus

Original Article

Abstract

This study was designed to explore the profile of immune cell subsets, including T, B, natural killer (NK), and NKT cells, in systemic lupus erythematosus (SLE) patients, and to determine their relationships with the clinical index and the effects of cyclophosphamide (CYC) and mycophenolate mofetil (MMF) treatment. SLE patients (n = 28) and age/sex-matched healthy controls (n = 28) were evaluated. The patients were equally divided into two treatment groups: intravenous drip (IVD) with CYC and prednisolone, and oral MMF and IVD with prednisolone. SLE peripheral blood samples were taken immediately prior to treatment and after 4 weeks of drug treatment. T, B, NK, and NKT cell subsets were measured by flow cytometry. Double-stranded DNA antibody and Sm antibody were detected by indirect immunofluorescence. Serum C3, C4, and C-reactive protein were determined by scatter turbidimetry. The percentages of CD3+CD4+ T, CD3–CD16CD56+ NK, and CD3+CD16CD56+ NKT cells and the CD4+/CD8+ ratio were significantly lower in SLE patients, while CD3+CD8+ T and CD3–CD19+ B cells were higher than the controls. The lymphocyte subsets were significantly correlated with the SLE disease activity index (SLEDAI) and complement factors (C3, C4). Four weeks of CYC or MMF treatment led to a significant increase in CD3+CD4+ T cells (P < 0.05). In addition, both CYC and MMF treatments led to increases in CD3+ T and CD3–CD16CD56+NKT cells and decreases in CD3–CD16CD56+ NK and CD3+CD8+ T cells, but these changes were not obvious. The significant correlation that exists between lymphocytes subsets and SLEDAI activity scores suggests that the lymphocyte subsets may reflect SLE severity. Our results indicate that both the traditional cyclophosphamide agent and the new mycophenolate mofetil agent can regulate the lymphocyte subsets and consequent abnormal immunity, suggesting that MMF, which is known to produce less side-effects than CYC, may be used as an effective treatment of SLE.

Keywords

Cyclophosphamide Lymphocyte subsets Mycophenolate mofetil Systemic lupus erythematosus 

Notes

Acknowledgement

This study was supported by grants from the National Natural Science Foundation of China (No. 30972610), Jilin Province Science and Technology Agency (Nos. 200705128 and 20110716), the Health Department Research Projects in Jilin Province (No. 2009Z054), the Cutting-edge Science and Interdisciplinary Innovation Projects of Jilin University, and Novartis Sebivo Post Approval Commitment study. We thank Medjaden Bioscience Limited for assisting in the preparation of this manuscript.

Disclosures

None.

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Copyright information

© Clinical Rheumatology 2012

Authors and Affiliations

  1. 1.Department of Rheumatology, First HospitalJilin UniversityChangchunChina
  2. 2.Department of Central Laboratory, the Second Part of First HospitalJilin UniversityChangchunChina

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