Clinical Rheumatology

, Volume 30, Issue 9, pp 1227–1233 | Cite as

Galectin-3 gene (LGALS3) +292C allele is a genetic predisposition factor for rheumatoid arthritis in Taiwan

  • Chung-Yi Hu
  • Sheng-Kai Chang
  • Chien-Sheng Wu
  • Wei-I Tsai
  • Ping-Ning Hsu
Original Article

Abstract

Galectin-3 is a beta-galactoside-binding lectin which is involved in modulating inflammation and apoptosis. Elevated expression of galectin-3 has been demonstrated in synovium of rheumatoid arthritis (RA). The aim of our study is to investigate the genetic polymorphisms of galectin-3 in association with RA. Polymorphisms of galectin-3 gene (LGALS3) were compared between 151 RA patients and 182 healthy subjects in Taiwan. Variants at two LGALS3 single nucleotide polymorphism (SNP) sites (rs4644 and rs4652, corresponding to LAGLS3 +191 and +292) were genotyped by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and sequence-specific oligonucleotide probe hybridization, respectively. The allelic carriage of LGALS3 +292C was increased in patients with RA (66.9% in RA vs. 52.7% in controls, odds ratio = 1.8, 95% confidence interval = 1.2–2.8, p = 0.009). These results implicate that the genetic polymorphisms in galectin-3 gene may contribute to development of RA.

Keywords

Galectin-3 Genetic polymorphism Rheumatoid arthritis 

Notes

Acknowledgments

This study was supported by grants from the National Science Council (NSC-94-2320-B-002-085), National Taiwan University Hospital (VN 9801), and National Health Research Institute (NHRI-EX95-9532SI), Taiwan. Polyclonal galectin-3 antibodies used in detecting serum galectin-3 were kindly provided by Dr. Liu FT.

Disclosures

None.

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Copyright information

© Clinical Rheumatology 2011

Authors and Affiliations

  • Chung-Yi Hu
    • 1
  • Sheng-Kai Chang
    • 1
  • Chien-Sheng Wu
    • 2
    • 3
  • Wei-I Tsai
    • 3
  • Ping-Ning Hsu
    • 3
    • 4
  1. 1.Department of Clinical Laboratory Sciences and Medical Biotechnology, College of MedicineNational Taiwan UniversityTaipeiRepublic of China
  2. 2.Department of Internal MedicineFar Eastern Memorial HospitalTaipeiRepublic of China
  3. 3.Department of Internal MedicineNational Taiwan University HospitalTaipeiRepublic of China
  4. 4.Graduate Institute of Immunology, College of MedicineNational Taiwan UniversityTaipeiRepublic of China

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