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Clinical Rheumatology

, Volume 23, Issue 1, pp 21–26 | Cite as

The significance of altered gelatinase expression in the synovium of patient with arthritic effusions

  • Ko-Hsiu Lu
  • Shun-Fa Yang
  • Shu-Chen Chu
  • Pei-Ni Chen
  • Ming-Chih Chou
  • Tang-Yan Hsiao
  • Yih-Shou HsiehEmail author
Original Article

Abstract

In this study we quantified the levels of matrix metalloproteinase-2 and 9 (MMP-2 and 9) in effusions and serial synovial cultures of patients with arthritis of the knee in order to investigate the correlations between MMP and cell counts in effusions as well as the possible roles of the synovium. In 49 patients with arthritis of the knee (series I) we examined the cell counts and the amounts of MMP-2 and 9 in 51 effusions. In 20 knee samples of series I of patients who received arthroscopy (series II), we examined the amounts of MMP-2 and 9 in effusions and serial synovial organ cultures. We also compared the gene expressions of MMP-2 and 9 and MT1-MMP in serial synovial cultures using RT-PCR. In series I, significantly more proMMP-9 appeared in effusions from the inflammatory group than in the non-inflammatory and hemorrhagic group (p <0.001). The levels of proMMP-9 correlated with the neutrophil counts in the effusions (p <0.001). In series II synovial cultures, the activities of latent and activated forms of MMP-2 and 9 in lesional areas were all higher than that in paralesional ones (p <0.05). In RT-PCR analysis, MMP-2, -9 and membrane type 1 MMP mRNA levels of lesional areas also showed increased expression. Our data suggest that the analysis of MMP-9 indicates the inflammatory condition of the joints and that additional synovectomy may be beneficial for patients with inflammatory synovitis, compared with non-inflammatory and hemorrhagic arthritis.

Keywords

Arthritis MMP-2 MMP-9 Neutrophils Synovectomy 

Abbreviations

ECM

Extracellular matrix

GA

Gouty arthritis

IDV

Integrated density value

MMP

Matrix metalloproteinases

OA

Osteoarthritis

PVNS

Pigmented villonodular synovitis

RA

Rheumatoid arthritis

SF

Synovial fluid

TIMP

Tissue inhibitors of metalloproteinases

TKA

Total knee arthroplasty

Notes

Acknowledgment

This work was supported by grant from the Research Section of Chung Shan Medical University (CSMU 90-OM-B-017) and National Science Council, Taiwan (NSC91–2314-B-040–019).

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Copyright information

© Clinical Rheumatology 2004

Authors and Affiliations

  • Ko-Hsiu Lu
    • 1
  • Shun-Fa Yang
    • 2
  • Shu-Chen Chu
    • 3
  • Pei-Ni Chen
    • 2
  • Ming-Chih Chou
    • 2
  • Tang-Yan Hsiao
    • 1
  • Yih-Shou Hsieh
    • 2
    Email author
  1. 1.Department of Orthopaedic SurgeryChung Shan Medical University HospitalTaichungTaiwan
  2. 2.Chung Shan Medical University, No. 110, Sec.1TaichungTaiwan
  3. 3.Department of Food ScienceChungtai Institute of Health Sciences and TechnologyTaichungTaiwan

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