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Molecules and Cells

, Volume 35, Issue 6, pp 543–549 | Cite as

The role of lysyl oxidase-like 2 in the odontogenic differentiation of human dental pulp stem cells

  • Joo-Hyun Kim
  • Eun-Hyang Lee
  • Hye-jeong Park
  • Eui-Kyun Park
  • Tae-Geon Kwon
  • Hong-In Shin
  • Je-Yoel ChoEmail author
Research Article

Abstract

Adult human dental pulp stem cells (hDPSCs) are a unique population of precursor cells those are isolated from postnatal dental pulp and have the ability to differentiate into a variety of cell types utilized for the formation of a reparative dentin-like complex. Using LC-MS/MS proteomics approaches, we identified the proteins secreted from the differentiating hDPSCs in mineralization media. Lysyl oxidase-like 2 (LOXL2) was identified as a protein that was down-regulated in the hDPSCs that differentiate into odontoblast-like cells. The role of LOXL2 has not been studied in dental pulp stem cells. LOXL2 mRNA levels were reduced in differentiating hDPSCs, whereas the levels of other LOX family members including LOX, LOXL1, LOXL3, and LOXL4, are increased. The protein expression and secretion levels of LOXL2 were also decreased during odontogenic differentiation. Recombinant LOXL2 protein treatment to hDPSCs resulted in a dose-dependent decrease in the early differentiation and the mineralization accompanying with the lower levels of odontogenic markers such as DSPP, DMP-1 and ALP. These results suggest that LOXL2 has a negative effect on the differentiation of hDPSCs and blocking LOXL2 can promote the hDPSC differentiation to odontoblasts.

Keywords

human dental pulp stem cells lysyl oxidase-like 2 odontogenic differentiation 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2013

Authors and Affiliations

  • Joo-Hyun Kim
    • 1
    • 2
  • Eun-Hyang Lee
    • 3
  • Hye-jeong Park
    • 1
  • Eui-Kyun Park
    • 2
  • Tae-Geon Kwon
    • 4
  • Hong-In Shin
    • 2
  • Je-Yoel Cho
    • 1
    Email author
  1. 1.Department of Veterinary Biochemistry, Brain Korea 21 and Research Institute for Veterinary Science, College of Veterinary MedicineSeoul National UniversitySeoulKorea
  2. 2.Department of Pathology, School of DentistryKyungpook National UniversityDaeguKorea
  3. 3.ProtAnBio, Co.SeoulKorea
  4. 4.Department of Oral and Maxillofacial Surgery, School of DentistryKyungpook National UniversityDaeguKorea

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