Molecules and Cells

, Volume 35, Issue 4, pp 348–354 | Cite as

Acteoside improves survival in cecal ligation and puncture-induced septic mice via blocking of high mobility group box 1 release

  • Eun Sun Seo
  • Bo Kang Oh
  • Jhang Ho Pak
  • Soon-Ho Yim
  • Sangilyandi Gurunathan
  • Young-Pil Kim
  • Kyung Jin Lee
Research Article
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Abstract

Acteoside, an active phenylethanoid glycoside, has been used traditionally as an anti-inflammatory agent. The molecular mechanism by which acteoside reduces inflammation was investigated in lipopolysaccharide (LPS)-induced Raw264.7 cells and in a mouse model of cecal ligation and puncture (CLP)-induced sepsis. In vitro, acteoside inhibits high mobility group box 1 (HMGB1) release and iNOS/NO production and induces heme oxygenase-1 (HO-1) expression in a concentration-dependent manner, while HO-1 siRNA antagonizes the inhibition of HMGB1 and NO. The effect of acteoside is inhibited by the p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580 and Nfr2 siRNA, indicating that acteoside induces HO-1 via p38 MAPK and NF-E2-related factor 2 (Nrf2). In vivo, acteoside increases survival and decreases serum and lung HMGB1 levels in CLP-induced sepsis. Overall, these results that acteoside reduces HMGB1 release and may be beneficial for the treatment of sepsis.

Keywords

acteoside heme oxygenase 1 high-mobility group box 1 nrf2 p38 Raw264.7 cell sepsis 
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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2013

Authors and Affiliations

  • Eun Sun Seo
    • 1
    • 2
  • Bo Kang Oh
    • 1
  • Jhang Ho Pak
    • 1
  • Soon-Ho Yim
    • 1
  • Sangilyandi Gurunathan
    • 3
  • Young-Pil Kim
    • 4
  • Kyung Jin Lee
    • 1
  1. 1.Department of MedicineUniversity of Ulsan College of Medicine, Asan Medical CenterSeoulKorea
  2. 2.Department of OptometryDong Shin UniversityNajuKorea
  3. 3.Department of Animal BiotechnologyKonkuk UniversitySeoulKorea
  4. 4.Department of Life ScienceHanyang UniversitySeoulKorea

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