Molecules and Cells

, Volume 33, Issue 4, pp 335–341

Extracellular HIV-1 Tat induces human beta-defensin-2 production via NF-kappaB/AP-1 dependent pathways in human B cells

  • Sung Mi Ju
  • Ah Ra Goh
  • Dong-Joo Kwon
  • Gi Soo Youn
  • Hyung-Joo Kwon
  • Yong Soo Bae
  • Soo Young Choi
  • Jinseu Park
Article

DOI: 10.1007/s10059-012-2287-0

Cite this article as:
Ju, S.M., Goh, A.R., Kwon, DJ. et al. Mol Cells (2012) 33: 335. doi:10.1007/s10059-012-2287-0

Abstract

Defensins, a family of antimicrobial peptides, are one of the first lines of host defense. Human beta-defensins (hBD) such as hBD-2 and -3 have anti-HIV activity. Previous studies have shown that HIV-1 virion can induce the expression of hBD, although the exact components of HIV-1 virion that are responsible for hBD expression have not yet been elucidated. In this study, we examined the effect of HIV-1 Tat on the expression of hBD in B cells. Stimulation of B cells with HIV-1 Tat protein significantly increased the mRNA and protein levels of hBD-2. HIV-1 Tat also induced the activation of a reporter gene for hBD-2 in a dose-dependent manner in B cells. Pretreatment of B cells with a JNK inhibitor suppressed HIV-1 Tat-induced hBD-2 expression. Pretreatment of B cells with AP-1 inhibitors or NF-κB inhibitors led to a decrease in HIV-1 Tat-induced protein and mRNA expression of hBD-2. Taken together, our results indicate that HIV-1 Tat can up-regulate the expression of hBD-2 via JNK-NF-κB/AP-1-dependent pathways in human B cells.

Keywords

AP-1 B cells beta-defensins HIV MAPK NF-κB Tat 

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2012

Authors and Affiliations

  • Sung Mi Ju
    • 3
  • Ah Ra Goh
    • 3
  • Dong-Joo Kwon
    • 3
  • Gi Soo Youn
    • 3
  • Hyung-Joo Kwon
    • 1
  • Yong Soo Bae
    • 2
  • Soo Young Choi
    • 3
  • Jinseu Park
    • 3
  1. 1.Department of Microbiology, College of MedicineHallym UniversityChunchonKorea
  2. 2.Department of Biological Science, College of Natural SciencesSungkyunkwan UniversitySuwonKorea
  3. 3.Department of Biomedical Science, Research Institute for Bioscience and BiotechnologyHallym UniversityChunchonKorea

Personalised recommendations