Molecules and Cells

, 32:123 | Cite as

The genome-wide expression profile of 1,2,3,4,6-penta-O-galloyl-β-D-glucose-treated MDA-MB-231 breast cancer cells: Molecular target on cancer metabolism

  • Woo Sik Yu
  • Soo-Jin Jeong
  • Ji-Hyun Kim
  • Hyo-Jung Lee
  • Hyo Sook Song
  • Min-Seok Kim
  • Eunjung Ko
  • Hyo-Jeong Lee
  • Jae-Ho Khil
  • Hyeung-Jin Jang
  • Young Chul Kim
  • Hyunsu Bae
  • Chang Yan Chen
  • Sung-Hoon Kim
Article
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Abstract

1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphenolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the significant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.

Keywords

cancer metabolism MDA-MB-231 microarray PGG real-time PCR 
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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2011

Authors and Affiliations

  • Woo Sik Yu
    • 1
  • Soo-Jin Jeong
    • 1
  • Ji-Hyun Kim
    • 1
  • Hyo-Jung Lee
    • 1
  • Hyo Sook Song
    • 1
  • Min-Seok Kim
    • 2
  • Eunjung Ko
    • 1
  • Hyo-Jeong Lee
    • 1
  • Jae-Ho Khil
    • 3
  • Hyeung-Jin Jang
    • 1
  • Young Chul Kim
    • 1
  • Hyunsu Bae
    • 1
  • Chang Yan Chen
    • 4
  • Sung-Hoon Kim
    • 1
  1. 1.Cancer Preventive Material Development Research Center, College of Oriental MedicineKyung Hee UniversitySeoulKorea
  2. 2.College of Dental MedicineTufts UniversityBostonUSA
  3. 3.College of Physical EducationKyung Hee UniversitySeoulKorea
  4. 4.Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA

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