Quantitative trait loci for cold tolerance of rice recombinant inbred lines in low temperature environments
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Low temperature is one of the major environmental stresses in rice cultivation in high-altitude and high-latitude regions. In this study, we cultivated a set of recombinant inbred lines (RIL) derived from Dasanbyeo (indica) / TR22183 (japonica) crosses in Yanji (high-latitude area), Kunming (high-altitude area), Chuncheon (cold water irrigation) and Suwon (normal) to evaluate the main effects of quantitative trait loci (QTL) and epistatic QTL (E-QTL) with regard to their interactions with environments for coldrelated traits. Six QTLs for spikelet fertility (SF) were identified in three cold treatment locations. Among them, four QTLs on chromosomes 2, 7, 8, and 10 were validated by several near isogenic lines (NILs) under cold treatment in Chuncheon. A total of 57 QTLs and 76 E-QTLs for nine cold-related traits were identified as distributing on all 12 chromosomes; among them, 19 QTLs and E-QTLs showed significant interactions of QTLs and environments (QEIs). The total phenotypic variation explained by each trait ranged from 13.2 to 29.1% in QTLs, 10.6 to 29.0% in EQTLs, 2.2 to 8.8% in QEIs and 1.0% to 7.7% in E-QTL × environment interactions (E-QEIs). These results demonstrate that epistatic effects and QEIs are important properties of QTL parameters for cold tolerance at the reproductive stage. In order to develop cold tolerant varieties adaptable to wide-ranges of cold stress, a strategy facilitating marker-assisted selection (MAS) is being adopted to accumulate QTLs identified from different environments.
Keywordscold tolerance near isogenic line quantitative trait loci rice RIL
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- Hill, J., Becker, H.C., and Tigerstedt, P.M.A. (1998). Quantitative and ecological aspects of plant breeding. Chapman & Hall, London, pp. 275.Google Scholar
- Javier, E.L., and Toledo, M.C. (2001). Finding genetic donors for cold tolerance the INGER gene pool. In ACIAR Proceedings 101; Increased Lowland Rice Production in the Mekong Region, S. Fukai and J. Basnayake (eds.), Australian Center for International Agricultural Research, GPO Box 1571, Canberra, ACT 2601, pp. 147–151.Google Scholar
- Jiang, W., Lee, J., Chu, S.H., Ham, T.H., Woo, M.O., Cho, Y.I., Chin, J.H., Han, L.Z., Xuan, Y., Yuan, D., et al. (2010). Genotype × environment interactions for chilling tolerance of rice recombinant inbred lines under different low temperature environments. Field Crops Res. 117, 226–236.CrossRefGoogle Scholar
- Kuroki, M., Saito, K., Matsuba, S., Yokogami, N., Shimizu, H., Ando, I., and Sato, Y. (2009). Quantitative trait locus analysis for cold tolerance at the booting stage in a rice cultivar, Hatsushizuku. Jpn. Agric. Res. Quart. 43, 115–121.Google Scholar
- Lee, M.H. (2001). Low temperature tolerance in rice: the Korean experience. In ACIAR Proceedings 101; Increased Lowland Rice Production in the Mekong Region, S. Fukai and J. Basnayake (eds.), Australian Center for International Agricultural Research, GPO Box 1571, Canberra, ACT 2601, pp. 138–146.Google Scholar
- Park, I.S., Park, S.K., Kim, S.L., Song, B.H., Jiang, W., Cho, Y.I., Koh, H.J., and Cho, Y.G. (2004). QTL analysis of agronomic traits and intracellular substances related to cold tolerance in rice (Oryza sativa L.). Korean J. Breed. 36, 9–19.Google Scholar
- Saito, K., Miura, K., Nagano, K., Hayano-Saito, Y., Araki, H., and Kato, A. (1995). Chromosomal location of quantitative trait loci for cool tolerance at the booting stage in rice variety ‘Norin-PL8’. Breed. Sci. 45, 337–340.Google Scholar
- SAS Institute Inc. (1999) SAS/Stat User’s Guide, Version 8.2.Google Scholar
- SAS institute, Inc., Cary, NC, USA. Satake, T. (1976). Sterility-type cold injury in paddy rice plants. In: Proceedings of the symposium on climate and rice. IRRI, Los Baños, Philippines, pp. 281–300.Google Scholar
- Suh, J.P., Jeung, J.U., Lee, J.I., Choi, Y.H., Yea, J.D., Virk, P.S., Mackill, D.J., and Jena, K.K. (2010). Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.). Theor. Appl. Genet. 120, 985–995.PubMedCrossRefGoogle Scholar
- Wu, R., and Garg, A. (2003). Engineering rice plant with trehaloseproducing genes improves tolerance to drought, salt and low temperature. ISB news report, March 2003. Covering agriculGoogle Scholar