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Molecules and Cells

, Volume 32, Issue 5, pp 451–457 | Cite as

Jolkinolide B from Euphorbia fischeriana Steud induces apoptosis in human leukemic U937 cells through PI3K/Akt and XIAP pathways

  • Jia-He WangEmail author
  • Yi-Jun Zhou
  • Xue Bai
  • Ping He
Article

Abstract

Jolkinolide B, a bioactive diterpene isolated from the roots of Euphorbia fischeriana Steud, is known to induce apoptosis in cancer cells. However, the molecular mechanism of its anti-cancer activity has not been fully elucidated. In the present study, we found that Jolkinolide B reduced cell viability and induced apoptosis in a dose- and timedependent manner in human leukemic U937. The induction of apoptosis was also accompanied by the downregulation of PI3K/Akt and the inhibitor of apoptosis protein (IAP) family proteins. Moreover, we observed that Jolkinolide B treatment resulted in activation of caspase-3 and -9, which may partly explain the anti-cancer activity of Jolkinolide B. Taken together, our study for the first time suggest that Jolkinolide B is able to enhance apoptosis of U937 cells, at least in part, through downregulation of PI3K/Akt and IAP family proteins. Moreover, triggering of caspase-3 and -9 activation mediated apoptotic induction.

Keywords

apoptosis caspase Euphorbia fischeriana Steud Jolkinolide B U937 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2011

Authors and Affiliations

  1. 1.Department of GeriatricsShengjing Hospital of China Medical UniversityShenyangP.R. China
  2. 2.Department of Endocrinology and Metabolismthe fourth affiliated hospital of China Medical UniversityShenyangP.R. China

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