ANK repeat-domain of SHN-1 Is indispensable for in vivo SHN-1 function in C. elegans
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Shank protein is one of the postsynaptic density (PSD) proteins which play a major role in proper localization of proteins at membranes. The shn-1, a homolog of Shank in Caenorhabditis elegans, is expressed in neurons, pharynx, intestine, vulva and sperm. We have previously reported a possible genetic interaction between Shank and IP3 receptor by examining shn-1 RNAi in IP3 receptor (itr-1) mutant background. In order to show the direct interaction of Shank and IP3 receptor as well as to show the direct in vivo function of Shank, we have characterized two different mutant alleles of shn-1, which have different deletions in the different domains. shn-1 mutants were observed for Ca2+-related behavioral defects with itr-1 mutants. We found that only shn-1 mutant defective in ANK repeat-domain showed significant defects in defecation, pharyngeal pumping and fertility. In addition, we found that shn-1 regulates defecation, pharyngeal pumping and probably male fertility with itr-1. Thus, we suggest that Shank ANK repeat-domain along with PDZ may play a crucial role in regulating Ca2+-signaling with IP3 receptor.
KeywordsANK repeat-domain defecation fertility IP3 receptor pharyngeal pumping Shank
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