Phylogenetic analysis and inflow route of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea
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Tomato yellow leaf curl virus (TYLCV) is a member of the genus Begomovirus of the family Geminiviridae, members of which are characterized by closed circular single-stranded DNA genomes of 2.7-2.8 kb in length, and include viruses transmitted by the Bemisia tabaci whitefly. No reports of TYLCV in Korea are available prior to 2008, after which TYLCV spread rapidly to most regions of the southern Korean peninsula (Gyeongsang-Do, Jeolla-Do and Jeju-Do). Fifty full sequences of TYLCV were analyzed in this study, and the AC1, AV1, IR, and full sequences were analyzed via the muscle program and bayesian analysis. Phylogenetic analysis demonstrated that the Korea TYLCVs were divided into two subgroups. The TYLCV Korea 1 group (Masan) originated from TYLCV Japan (Miyazaki) and the TYLCV Korea 2 group (Jeju/Jeonju) from TYLCV Japan (Tosa/Haruno). A B. tabaci phylogenetic tree was constructed with 16S rRNA and mitochondria cytochrome oxidase I (MtCOI) sequences using the muscle program and MEGA 4.0 in the neighbor-joining algorithm. The sequence data of 16S rRNA revealed that Korea B. tabaci was closely aligned to B. tabaci isolated in Iran and Nigeria. The Q type of B. tabaci, which was originally identified as a viruliferous insect in 2008, was initially isolated in Korea as a non-viruliferous insect in 2005. Therefore, we suggest that two TYLCV Japan isolates were introduced to Korea via different routes, and then transmitted by native B. tabaci.
Keywordsbegomovirus Bemisia tabaci phylogeny tomato TYLCV
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- Boykin, L.M., Shatters, R.G., Jr., Rosell, R.C., McKenzie, C.L., Bagnall, R.A., De Barro, P., and Frohlich, D.R. (2007). Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial COI DNA sequences. Mol. Phylogenet. Evol. 44, 1306–1319.CrossRefPubMedGoogle Scholar
- Davino, S., Napoli, C., Dellacroce, C., Miozzi, L., Noris, E., Davino, M., and Accotto, G.P. (2009). Two new natural begomovirus recombinants associated with the tomato yellow leaf curl disease co-exist with parental viruses in tomato epidemics in Italy. Virus Res. 143, 15–23.CrossRefPubMedGoogle Scholar
- Everett, K.D., Thao, M., Horn, M., Dyszynski, G.E., and Baumann, P. (2005). Novel chlamydiae in whiteflies and scale insects: endosymbionts ‘Candidatus Fritschea bemisiae’ strain Falk and ‘Candidatus Fritschea eriococci’ strain Elm. Int. J. Syst. Evol. Microbiol. 55, 1581–1587.CrossRefPubMedGoogle Scholar
- Kim, M.I., Baek, J.Y., Kim, M.J., Jeong, H.C., Kim, K.G., Bae, C.H., Han, Y.S., Jin, B.R., and Kim, I. (2009). Complete nucleotide sequence and organization of the mitogenome of the red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) and comparison with other lepidopteran insects. Mol. Cells 28, 347–363.CrossRefPubMedGoogle Scholar
- Matsuda, N., Sharma, P., Bajet, N.B., and Ikegami, M. (2008). Molecular characterization of a new strain of tomato leaf curl Philippines virus and its associated satellite DNAbeta molecule: further evidence for natural recombination amongst begomoviruses. Arch. Virol. 153, 961–967.CrossRefPubMedGoogle Scholar
- Shatters, R.G., Jr., Powell, C.A., Boykin, L.M., Liansheng, H., and McKenzie, C.L. (2009). Improved DNA barcoding method for Bemisia tabaci and related Aleyrodidae: development of universal and Bemisia tabaci biotype-specific mitochondrial cytochrome c oxidase I polymerase chain reaction primers. J. Econ. Entomol. 102, 750–758.CrossRefPubMedGoogle Scholar