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Molecules and Cells

, Volume 30, Issue 2, pp 137–141 | Cite as

Engineering of flavonoid O-methyltransferase for a novel regioselectivity

  • Eun Ji Joe
  • Bong-Gyu Kim
  • Byoung-Chan An
  • Youhoon Chong
  • Joong-Hoon Ahn
Article

Abstract

An O-methyltransferase isolated from poplar, POMT7, was identified as a flavone 7-O-methyltransferase. In order to generate a mutant of POMT-7 having a novel regioselectivity, we conducted an error-prone polymerase chain reaction. More than 100 mutants were screened and one of the mutants (POMT-M1) Asp257Gly, methylated the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The mutation changed asparagine residue at the position of 257 into glycine. The kinetic parameters showed that the wild type POMT7 was better activity toward kaempferol and quercetin than the POMT7-M1. Using E. coli transformant expressing POMT7-M1, 58 μM of 3, 7-O-dimethylquercetin and 70 μM of 3, 7-O-dimethylkaempferol from 100 μM of corresponding substrate were synthesized successfully.

Keywords

flavonoid O-methyltransferase regioselectivity 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2010

Authors and Affiliations

  • Eun Ji Joe
    • 1
  • Bong-Gyu Kim
    • 1
  • Byoung-Chan An
    • 1
  • Youhoon Chong
    • 1
  • Joong-Hoon Ahn
    • 1
  1. 1.Department of Bioscience and Biotechnology, Bio/Molecular Informatics CenterKonkuk UniversitySeoulKorea

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