Molecules and Cells

, Volume 29, Issue 6, pp 567–574 | Cite as

A cyclophilin A CPR1 overexpression enhances stress acquisition in Saccharomyces cerevisiae

  • Il-Sup Kim
  • Hyun-Young Kim
  • Sun-Young Shin
  • Young-Saeng Kim
  • Dong Hee Lee
  • Kyung Moc Park
  • Ho-Sung Yoon


Cyclophilins are conserved cis-trans peptidyl-prolyl isomerase that are implicated in protein folding and function as molecular chaperones. We found the expression of cyclophilin A, Cpr1, changes in response to exposure to yeast Saccharomyces cerevisiae to abiotic stress conditions. The effect of Cpr1 overexpression in stress responses was therefore examined. The CPR1 gene was cloned to the yeast expression vector pVTU260 under regulation of an endogenous alcohol dehydrogenase (ADH) promoter. The overexpression of Cpr1 drastically increased cell viability of yeast in the presence of stress inducers, such as cadmium, cobalt, copper, hydrogen peroxide, tert-butyl hydroperoxide (t-BOOH), and sodium dodecyl sulfate (SDS). The Cpr1 expression also enhanced the cell rescue program resulting in a variety of antioxidanr enzymes including thioredoxin system (particularly, thioredoxin peroxidase), metabolic enzymes (glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase), and molecular chaperones (Hsp104, Hsp90, Hsp60 and Hsp42). Thus, our study illustrates the importance of Cpr1 as a molecular chaperone that improves cellular stress responses through collaborative relationships with other proteins when yeast cells are exposed to adverse conditions, and it also premises the improvement of yeast strains.


cell rescue proteins cyclophilin A protein expression stress tolerance Saccharomyces cerevisiae 


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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2010

Authors and Affiliations

  • Il-Sup Kim
    • 1
  • Hyun-Young Kim
    • 1
  • Sun-Young Shin
    • 1
  • Young-Saeng Kim
    • 1
  • Dong Hee Lee
    • 2
  • Kyung Moc Park
    • 2
  • Ho-Sung Yoon
    • 1
  1. 1.Department of BiologyKyungpook National UniversityDaeguKorea
  2. 2.Genomine Inc.PohangKorea

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