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Molecules and Cells

, Volume 29, Issue 1, pp 85–91 | Cite as

Ca2+ signaling induced by sphingosine 1-phosphate and lysophosphatidic acid in mouse B cells

  • Joo Hyun Nam
  • Dong Hun Shin
  • Jung Eun Min
  • Sang-Kyu Ye
  • Ju-Hong Jeon
  • Sung Joon Kim
Article

Abstract

Lysophospholipids (LPLs) such as lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are chemotactic for lymphocytes, and increases of in cytosolic [Ca2+] signal the regulation of lymphocyte activation and migration. Here, the authors investigated the effects of LPA and S1P on [Ca2+]c in mouse B cell lines (WEHI-231 and Bal-17) and primary B cells isolated from mouse spleen and bone marrow, and focused on the modulation of store-operated Ca2+ entry (SOCE) by LPLs. In Bal-17 (a mature B cell line) both LPA and S1P induced a transient [Ca2+]c increase via a phospholipase C pathway. In addition, pretreatment with LPLs was found to augment thapsigargin-induced SOCE in Bal-17 cells. However, in WEHI-231 (an immature B cell line) LPLs had no significant effect on [Ca2+]c or SOCE. Furthermore, in freshly isolated splenic B cells (SBCs) and bone marrow B cells (BMBCs), LPLs induced only a small increase in [Ca2+]c. Interestingly, however, pretreatment with LPLs markedly increased SOCE in primary B cells, and this augmentation was more prominent in BMBCs than SBCs. The unidirectional influx of Ca2+ was measured using Ba2+ as a surrogate ion. Similarly, Ba2+ influx was also found to be markedly increased by LPLs in SBCs and BMBCs. Summarizing, LPLs were found to strongly augment SOCE-mediated Ca2+-signaling in mouse B cells. However, unlike the mature Bal-17 cell line, PLC-dependent Ca2+ release was insignificant in primary B cells and inWEHI-231.

Keywords

B cell Ca2+ signal lysophosphatidic acid sphingosine 1-phosphate store-operated Ca2+ influx 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2010

Authors and Affiliations

  • Joo Hyun Nam
    • 1
    • 2
  • Dong Hun Shin
    • 1
  • Jung Eun Min
    • 1
  • Sang-Kyu Ye
    • 3
    • 4
  • Ju-Hong Jeon
    • 1
  • Sung Joon Kim
    • 1
    • 3
    • 5
  1. 1.Department of PhysiologySeoul National University College of MedicineSeoulKorea
  2. 2.Infection Research InstituteSeoul National University College of MedicineSeoulKorea
  3. 3.Ischemic/Hypoxic Disease InstituteSeoul National University College of MedicineSeoulKorea
  4. 4.Department of PharmacologySeoul National University College of MedicineSeoulKorea
  5. 5.Kidney Research InstituteSeoul National University College of MedicineSeoulKorea

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