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Molecules and Cells

, Volume 29, Issue 1, pp 71–76 | Cite as

Non-specific phytohormonal induction of AtMYB44 and suppression of jasmonate-responsive gene activation in Arabidopsis thaliana

  • Choonkyun Jung
  • Jae Sung Shim
  • Jun Sung Seo
  • Han Yong Lee
  • Chung Ho Kim
  • Yang Do Choi
  • Jong-Joo CheongEmail author
Article

Abstract

The Arabidopsis thaliana transcription factor gene AtMYB44 was induced within 10 min by treatment with methyl jasmonate (MeJA). Wound-induced expression of the gene was observed in local leaves, but not in distal leaves, illustrating jasmonate-independent induction at wound sites. AtMYB44 expression was not abolished in Arabidopsis mutants insensitive to jasmonate (coi1), ethylene (etr1), or abscisic acid (abi3-1) when treated with the corresponding hormones. Moreover, various growth hormones and sugars also induced rapid AtMYB44 transcript accumulation. Thus, AtMYB44 gene activation appears to not be induced by any specific hormone. MeJA-induced activation of jasmonate-responsive genes such as JR2, VSP, LOXII, and AOS was attenuated in transgenic Arabidopsis plants overexpressing the gene (35S:AtMYB44), but significantly enhanced in atmyb44 knockout mutants. The 35S:MYB44 and atmyb44 plants did not show defectiveness in MeJA-induced primary root growth inhibition, indicating that the differences in jasmonate-responsive gene expression observed was not due to alterations in the jasmonate signaling pathway. 35S:AtMYB44 seedlings exhibited slightly elevated chlorophyll levels and less jasmonate- induced anthocyanin accumulation, demonstrating suppression of jasmonate-mediated responses and enhancement of ABA-mediated responses. These observations support the hypothesis of mutual antagonistic actions between jasmonate- and abscisic acid-mediated signaling pathways.

Keywords

Arabidopsis AtMYB44 jasmonate transcription factor transgenic plant 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2010

Authors and Affiliations

  • Choonkyun Jung
    • 1
    • 3
  • Jae Sung Shim
    • 1
  • Jun Sung Seo
    • 1
  • Han Yong Lee
    • 1
  • Chung Ho Kim
    • 2
  • Yang Do Choi
    • 1
  • Jong-Joo Cheong
    • 1
    Email author
  1. 1.Department of Agricultural Biotechnology and Center for Agricultural BiomaterialsSeoul National UniversitySeoulKorea
  2. 2.Department of Food and NutritionSeowon UniversityChongjuKorea
  3. 3.Laboratory of Plant Molecular BiologyThe Rockefeller UniversityNew YorkUSA

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