Molecules and Cells

, Volume 10, Issue 6, pp 647–653 | Cite as

Identification, Expression, and Nuclear Location of Murine Mage-b2 Protein, a Tumor-associated Antigen

  • Jung-Hee Lee
  • Bong-Whan Sung
  • Hyun-Joo Youn
  • Joo-Hung Park
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Abstract

MAGE-1, which was originally identified by reacting with cytolytic T lymphocytes derived from the blood of melanoma patients, is a member of a gene family consisting of 17 structurally related genes. The MAGE genes are expressed only in the testis among normal tissues and in a number of human tumors of various histological types. Murine MAGE (also called SMAGE or Mage) genes were found in a study aimed at detecting mouse genes homologous to human MAGE genes. However, the biological functions of MAGE and Mage are currently unknown. To understand the biological functions of Mage, in the present study a recombinant SMAGE2 (Mage-b2) protein of 43 kDa was produced and monoclonal antibodies reactive with Mage-b2 protein were generated. One monoclonal antibody, smpG4A, specifically recognized a 43 kDa protein in lysates of Mage-b2 mRNA-positive sarcoma cells and of the testis. Immunohistochemistry showed that Mage-b2 is located in the nucleus of Mage-b2 mRNA-positive sarcoma cells. These results should contribute to understanding the biological functions of Mage.

Keywords

MAGE Mage-b2 SMAGE Tumor-associated Antigen 

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Copyright information

© Springer-Verlag Singapore Pte. Ltd 2000

Authors and Affiliations

  • Jung-Hee Lee
    • 1
  • Bong-Whan Sung
    • 2
  • Hyun-Joo Youn
    • 2
  • Joo-Hung Park
    • 1
  1. 1.Department of BiologyChangwon National UniversityChangwonKorea
  2. 2.Department of MicrobiologyInje UniversityKimhaeKorea

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