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neurogenetics

, Volume 10, Issue 3, pp 217–228 | Cite as

Interaction of the SPG21 protein ACP33/maspardin with the aldehyde dehydrogenase ALDH16A1

  • Michael C. HannaEmail author
  • Craig BlackstoneEmail author
Original Article

Abstract

Mast syndrome (SPG21) is an autosomal-recessive complicated form of hereditary spastic paraplegia characterized by dementia, thin corpus callosum, white matter abnormalities, and cerebellar and extrapyramidal signs in addition to spastic paraparesis. A nucleotide insertion resulting in premature truncation of the SPG21 gene product acidic cluster protein 33 (ACP33)/maspardin underlies this disorder, likely causing loss of protein function. However, little is known about the function of maspardin. Here, we report that maspardin localizes prominently to cytoplasm as well as to membranes, possibly at trans-Golgi network/late endosomal compartments. Immunoprecipitation of maspardin with identification of coprecipitating proteins by mass spectrometry revealed the aldehyde dehydrogenase ALDH16A1 as an interacting protein. This interaction was confirmed using overexpressed proteins as well as by fusion protein pull down experiments, and these proteins colocalized in cells. Further studies of the function of ALDH16A1 and the role of the maspardin–ALDH16A1 interaction in neuronal cells may clarify the cellular pathogenesis of Mast syndrome.

Keywords

Aldehyde dehydrogenase ACP33 CD4 Spastic paraplegia Endocytosis 

Notes

Acknowledgments

The authors wish to thank Dr. Howard Jaffe (NINDS Protein/Peptide Sequencing Facility) for mass spectrometry, James Nagle and Debbie Kauffman (NINDS DNA Sequencing Facility) for DNA sequencing, and Henri Jupille and Julia Stadler for technical assistance. This study was supported by the Intramural Research Program of the NIH, National Institutes of Neurological Disorders and Stroke.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Department of Biological and Environmental SciencesTexas A & M University-CommerceCommerceUSA
  2. 2.Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and StrokeNational Institutes of HealthBethesdaUSA

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