Advertisement

Journal of Artificial Organs

, Volume 18, Issue 3, pp 257–263 | Cite as

Effects of increased surface coverage of polyvinylpyrrolidone over a polysulfone hemofilter membrane on permeability and cell adhesion during continuous hemofiltration

  • Kenichi KokuboEmail author
  • Yoshitaka Kurihara
  • Hiroshi Tsukao
  • Naoko Maruyama
  • Kozue Kobayashi
  • Toshihiro Shinbo
  • Minoru Hirose
  • Hirosuke Kobayashi
Original Article Artificial Kidney / Dialysis

Abstract

The purpose of the present study was to evaluate the adhesiveness of blood cells and the solute removal performance change of modified polysulfone membranes which have increased polyvinylpyrrolidone (PVP) coverage over their surface. Continuous hemofiltration (CHF) experiments for 24 h were carried out using an ex vivo hemofilter evaluation system to compare a modified polysulfone hemofilter (SHG) with the conventional polysulfone hemofilter (SH). The 25 and 50 % cutoff values of the sieving coefficient of dextran after CHF and the protein concentration in the filtrate was higher in SHG, indicating that less fouling occurred in the SHG membrane. Adhesion of blood cells after 24 h of CHF was significantly higher in the case of SH than in the case of SHG. Blood cell adhesion and membrane fouling were reduced with the use of a polysulfone membrane modified with increased PVP coverage over the surface.

Keywords:

Continuous hemofiltration Polyvinylpyrrolidone Sieving coefficient Cell adhesion 

Notes

Acknowledgments

The authors thank Hidetoshi Kozawa, Makiko Miyata and Hisako Miyazaki of Medical devices Department, Toray Industry Inc. and Hiroyuki Sugaya and Yoshiyuki Ueno of Advanced Material Research Labs, Toray Industry Inc. for their technical assistance and useful discussion regarding this study.

Conflict of interest

H. Kobayashi and K. Kokubo received research grants from Toray Industry Inc. and Toray Medical Co., Ltd. The other authors declare that they have no conflict of interest.

References

  1. 1.
    Ricci Z, Ronco C. Timing, dose and mode of dialysis in acute kidney injury. Curr Opin Crit Care. 2011;17:556–61.CrossRefPubMedGoogle Scholar
  2. 2.
    Uchino S. Choice of therapy and renal recovery. Crit Care Med. 2008;36:S238–42.CrossRefPubMedGoogle Scholar
  3. 3.
    Glassford NJ, Bellomo R. Acute kidney injury: how can we facilitate recovery? Curr Opin Crit Care. 2011;17:562–8.CrossRefPubMedGoogle Scholar
  4. 4.
    Davenport A. The coagulation system in the critically ill patient with acute renal failure and the effect of an extracorporeal circuit. Am J Kidney Dis. 1997;30:S20–7.CrossRefPubMedGoogle Scholar
  5. 5.
    Keller F, Seemann J, Preuschof L, Offermann G. Risk factors of system clotting in heparin-free haemodialysis. Nephrol Dial Transplant. 1990;5:802–7.CrossRefPubMedGoogle Scholar
  6. 6.
    Baldwin I. Factors affecting circuit patency and filter ‘life’. Contrib Nephrol. 2007;156:178–84.CrossRefPubMedGoogle Scholar
  7. 7.
    Schetz M. Anticoagulation for continuous renal replacement therapy. Curr Opin Anaesthesiol. 2001;14:143–9.CrossRefPubMedGoogle Scholar
  8. 8.
    Bellomo R, Ronco C. Circulation of the continuous artificial kidney: blood flow, pressures, clearances and the search for the best. Blood Purif. 1997;15:354–65.CrossRefPubMedGoogle Scholar
  9. 9.
    Uchino S, Fealy N, Baldwin I, Morimatsu H, Bellomo R. Pre-dilution vs. post-dilution during continuous veno-venous hemofiltration: impact on filter life and azotemic control. Nephron Clin Pract. 2003;94:c94–8.CrossRefPubMedGoogle Scholar
  10. 10.
    Nurmohamed SA, Jallah BP, Vervloet MG, Beishuizen A, Groeneveld AB. Predilution versus postdilution continuous venovenous hemofiltration: no effect on filter life and azotemic control in critically ill patients on heparin. ASAIO J. 2011;57:48–52.CrossRefPubMedGoogle Scholar
  11. 11.
    Krouzecky A, Chvojka J, Sykora R, Radej J, Karvunidis T, Novak I, Ruzicka J, Petrankova Z, Benes J, Bolek L, Matejovic M. Regional cooling of the extracorporeal blood circuit: A novel anticoagulation approach for renal replacement therapy? Intensive Care Med. 2009;35:364–70.CrossRefPubMedGoogle Scholar
  12. 12.
    Bowry SK, Ronco C. Surface topography and surface elemental composition analysis of helixone, a new high-flux polysulfone dialysis membrane. Int J Artif Organs. 2001;24:757–64.PubMedGoogle Scholar
  13. 13.
    Ochoa N, Prádanos P, Palacio L, Pagliero C, Marchese J, Hernández A. Pore size distributions based on afm imaging and retention of multidisperse polymer solutes: characterisation of polyethersulfone uf membranes with dopes containing different pvp. J Membr Sci. 2001;187:227–37.CrossRefGoogle Scholar
  14. 14.
    Hayama M, Yamamoto K, Kohori F, Uesaka T, Ueno Y, Sugaya H, Itagaki I, Sakai K. Nanoscopic behavior of polyvinylpyrrolidone particles on polysulfone/polyvinylpyrrolidone film. Biomaterials. 2004;25:1019–28.CrossRefPubMedGoogle Scholar
  15. 15.
    Koga S, Yakushiji T, Matsuda M, Yamamoto K, Sakai K. Functional-group analysis of polyvinylpyrrolidone on the inner surface of hollow-fiber dialysis membranes, by near-field infrared microspectroscopy. J Membr Sci. 2010;355:208–13.CrossRefGoogle Scholar
  16. 16.
    Zou Y, Lai BF, Kizhakkedathu JN, Brooks DE. Inhibitory effect of hydrophilic polymer brushes on surface-induced platelet activation and adhesion. Macromol Biosci. 2010;10:1432–43.CrossRefPubMedGoogle Scholar
  17. 17.
    Tsunoda N, Kokubo K, Sakai K, Fukuda M, Miyazaki M, Hiyoshi T. Surface roughness of cellulose hollow fiber dialysis membranes and platelet adhesion. Asaio J. 1999;45:418–23.CrossRefPubMedGoogle Scholar
  18. 18.
    Nojiri C, Park KD, Okano T, Kim SW. In vivo protein adsorption onto polymers: a transmission electron microscopic study. ASAIO Trans. 1989;35:357–61.CrossRefPubMedGoogle Scholar

Copyright information

© The Japanese Society for Artificial Organs 2015

Authors and Affiliations

  • Kenichi Kokubo
    • 1
    • 2
    Email author
  • Yoshitaka Kurihara
    • 1
  • Hiroshi Tsukao
    • 1
  • Naoko Maruyama
    • 1
    • 3
  • Kozue Kobayashi
    • 1
    • 2
  • Toshihiro Shinbo
    • 2
  • Minoru Hirose
    • 1
    • 2
  • Hirosuke Kobayashi
    • 1
    • 2
  1. 1.Kitasato University Graduate School of Medical SciencesSagamiharaJapan
  2. 2.Kitasato University School of Allied Health SciencesSagamiharaJapan
  3. 3.Faculty of Health and Medical CareSaitama Medical UniversitySaitamaJapan

Personalised recommendations