Journal of Artificial Organs

, Volume 16, Issue 1, pp 59–65

Implantation study of small-caliber “biotube” vascular grafts in a rat model

  • Masashi Yamanami
  • Hatsue Ishibashi-Ueda
  • Akihide Yamamoto
  • Hidehiro Iida
  • Taiji Watanabe
  • Keiichi Kanda
  • Hitoshi Yaku
  • Yasuhide Nakayama
Original Article

Abstract

We developed autologous vascular grafts, called “biotubes,” by simple and safe in-body tissue architecture technology, which is a practical concept of regenerative medicine, without using special sterile conditions or complicated in vitro cell treatment processes. In this study, biotubes of extremely small caliber were first auto-implanted to rat abdominal aortas. Biotubes were prepared by placing silicone rods (outer diameter 1.5 mm, length 30 mm) used as a mold into dorsal subcutaneous pouches in rats for 4 weeks. After argatroban coating, the obtained biotubes were auto-implanted to abdominal aortas (n = 6) by end-to-end anastomosis using a custom-designed sutureless vascular connecting system under microscopic guidance. Graft status was evaluated by contrast-free time-of-flight magnetic resonance angiography (TOF-MRA). All grafts were harvested at 12 weeks after implantation. The patency rate was 66.7 % (4/6). MRA showed little stenosis and no aneurysmal dilation in all biotubes. The original biotube had wall thickness of about 56.2 ± 26.5 μm at the middle portion and mainly random and sparse collagen fibers and fibroblasts. After implantation, the wall thickness was 235.8 ± 24.8 μm. In addition, native-like vascular structure was regenerated, which included (1) a completely endothelialized luminal surface, (2) a mesh-like elastin fiber network, and (3) regular circumferential orientation of collagen fibers and α-SMA positive cells. Biotubes could be used as small-caliber vascular prostheses that greatly facilitate the healing process and exhibit excellent biocompatibility in vascular regenerative medicine.

Keywords

Biotube Vascular grafts Autologous tissue In vivo tissue engineering Connective tissue 

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Copyright information

© The Japanese Society for Artificial Organs 2012

Authors and Affiliations

  • Masashi Yamanami
    • 1
    • 2
  • Hatsue Ishibashi-Ueda
    • 3
  • Akihide Yamamoto
    • 4
    • 5
  • Hidehiro Iida
    • 4
    • 5
  • Taiji Watanabe
    • 1
    • 2
  • Keiichi Kanda
    • 2
  • Hitoshi Yaku
    • 2
  • Yasuhide Nakayama
    • 1
  1. 1.Division of Medical Engineering and MaterialsNational Cerebral and Cardiovascular Center Research InstituteSuitaJapan
  2. 2.Department of Cardiovascular SurgeryKyoto Prefectural University of MedicineKyotoJapan
  3. 3.Department of PathologyNational Cerebral and Cardiovascular CenterOsakaJapan
  4. 4.Department of Biomedical ImagingNational Cerebral and Cardiovascular Center Research InstituteOsakaJapan
  5. 5.Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of MedicineOsaka UniversityOsakaJapan

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