Journal of Artificial Organs

, Volume 9, Issue 3, pp 185–191 | Cite as

Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells

  • Daisuke Murakami
  • Masayuki Yamato
  • Kohji Nishida
  • Takeshi Ohki
  • Ryo Takagi
  • Joseph Yang
  • Hideo Namiki
  • Teruo Okano
ORIGINAL ARTICLE

Abstract

To exclude bacteria- or animal-derived factors from cultured fabrication of transplantable epithelial cell sheets, primary human oral mucosal epithelial cells were seeded on temperature-responsive culture inserts having submicron-scale pores. Supplying culture medium containing human autologous serum to both apical and basal sides of human epithelial cells allows these cells to grow to confluence. These proliferating cells created stratified epithelial layers even when 3T3 feeder layers and fetal bovine serum were eliminated from culture. Normal keratin expression profiles were obtained with these cells, and basal and midlayer cells expressed p63, a putative stem/progenitor marker. These results suggest that temperature-responsive culture inserts can be useful in clinical settings that require the exclusion of xenogeneic factors.

Key words

Cell culture Epithelial cell Stem cell Thermally responsive material 

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Copyright information

© The Japanese Society for Artificial Organs 2006

Authors and Affiliations

  • Daisuke Murakami
    • 1
    • 2
  • Masayuki Yamato
    • 2
  • Kohji Nishida
    • 3
  • Takeshi Ohki
    • 2
    • 4
  • Ryo Takagi
    • 2
  • Joseph Yang
    • 2
  • Hideo Namiki
    • 1
  • Teruo Okano
    • 2
  1. 1.Graduate School of Science and EngineeringWaseda UniversityTokyoJapan
  2. 2.Institute of Advanced Biomedical Engineering and ScienceTokyo Women's Medical UniversityTokyoJapan
  3. 3.Department of Ophthalmology and Visual ScienceTohoku University Graduate School of MedicineSendaiJapan
  4. 4.Department of SurgeryInstitute of Gastroenterology, Tokyo Women's Medical UniversityTokyoJapan

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