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Optical Review

, Volume 6, Issue 3, pp 268–271 | Cite as

In Vitro Monitoring of Live Cardiomyocytes Dynamics by a Scanning Near Field Optical Microscope Setup

  • Ruggero Micheletto
  • Morgan Denyer
  • Martin Scholl
  • Ken Nakajima
  • Masahiko Hara
  • Wolfgang Knoll
5th International Conference on NEAR FIELD OPTICS and RELATED TECHNOLOGIES (NFO-5)

Abstract

We describe an original scanning near field optical microscope setup developed to examine rhythmically beating cardiac myocytes fully immersed in culture media. Scans could be halted at any point to record localized contraction profiles. Contractions could be detected with high sub nanometric vertical sensitivity and changed shape dramatically within adjacent sub micron-sized areas. We believe that the spatial dependency of contractions arises because of system’s ability to resolve the dynamic behavior of individual sub membrane actin bundles. Our results, combining imaging and real time recording in localized areas, reveal a new, non-invasive method for studying sub micron morphological activity in live biological samples.

Key words

SNOM near-field scanning microscope dynamics cardiomyocytes beating heart contraction 

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References

  1. 1.
    E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, R. L. Kostelak: Science 251 (1991) 1468.Google Scholar
  2. 2.
    M. Ohtsu: OPTOELECTRONIC Dev. Tech. 10 (1995) 147.Google Scholar
  3. 3.
    A. Shrier and J. R. Clay: Nature 283 (1980) 670.CrossRefPubMedGoogle Scholar
  4. 4.
    S. Mononobe, M. Naya, T. Saiki and M. Ohtsu: Appl. Opt. 36 (1997) 1496.Google Scholar
  5. 5.
    T. Saiki, S. Mononobe, M. Ohtsu, N. Saito and K. Kusano: Appl. Phys. Lett. 68 (1996) 2612.CrossRefGoogle Scholar
  6. 6.
    J. H. Rose, M. R. Kaufmann, S. A. Wickline, C. S. Hall and J. G. Miller: J. Acoust Soc. Am. 97 (1995) 656.PubMedGoogle Scholar
  7. 7.
    J. Meunier and M. Bertrand: IEEE Trans. Biomed. Eng. 42 (1995) 403.CrossRefPubMedGoogle Scholar
  8. 8.
    M. F. Arnsdorf and R. Lal: Proc. SPIE 1778 (1992) 112.Google Scholar
  9. 9.
    M. Courtois, S. Khatami, E. Fantini, P. Athias, P. Mielle and A. Grynberg: Am. J. Physiol. 262 (1992) H451.PubMedGoogle Scholar

Copyright information

© The Optical Society of Japan 1999

Authors and Affiliations

  • Ruggero Micheletto
    • 1
  • Morgan Denyer
    • 1
  • Martin Scholl
    • 1
  • Ken Nakajima
    • 1
  • Masahiko Hara
    • 1
  • Wolfgang Knoll
    • 2
  1. 1.Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN)SaitamaJapan
  2. 2.Max-Planck Institute for Polymer ResearchMainzGermany

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