Optical Review

, Volume 14, Issue 4, pp 161–164 | Cite as

A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer

  • Toshiki Yasokawa
  • Ichirou Ishimaru
  • Masahiro Kondo
  • Shigeki Kuriyama
  • Tsutomu Masaki
  • Kaoru Takegawa
  • Naotaka Tanaka
Letter

Abstract

This paper describes a method for measuring the three-dimensional (3D) refractive-index distribution in a single cell. The method can be used to observe the distribution of cell components without fluorescence staining. The two-dimensional optical path length distributions from multiple directions are obtained by non-contact rotation of the cell. These optical path lengths are converted into the line integrals of the refractive index, and the 3D refractive-index distribution is reconstructed by means of computed tomography. The refractive-index distribution in a breast cancer cell can be measured using a phase-shifting Mach—Zehnder interferometer in conjunction with proximal two-beam optical tweezers.

Key words

cell refractive index three-dimensional imaging computed tomography rotation optical tweezers light pressure phase shift interferometer Mach—Zehnder interferometer 

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Copyright information

© The Optical Society of Japan 2007

Authors and Affiliations

  • Toshiki Yasokawa
    • 1
  • Ichirou Ishimaru
    • 1
  • Masahiro Kondo
    • 1
  • Shigeki Kuriyama
    • 2
  • Tsutomu Masaki
    • 2
  • Kaoru Takegawa
    • 3
  • Naotaka Tanaka
    • 3
  1. 1.Faculty of EngineeringKagawa UniversityHayashichoJapan
  2. 2.Faculty of MedicineKagawa UniversityKita-gunJapan
  3. 3.Faculty of AgricultureKagawa UniversityKita-gunJapan

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