Optical Review

, Volume 12, Issue 5, pp 415–419 | Cite as

Finding of Optimal Calcium Ion Probes for Fluorescence Lifetime Measurement

  • Keisuke Yoshiki
  • Hiroki Azuma
  • Kazuhiko Yoshioka
  • Mamoru Hashimoto
  • Tsutomu Araki


We have investigated the fluorescence lifetime properties of 8 calcium ion probes, calcium-green-1, calcium green-2, calcium green-5N, calcium orange, oregon green 488 BAPTA-6F, fluo-3, fluo-4, and fluo-5N. We found that the decay time of calcium green-5N varied more sensitively with calcium concentration than calcium green-1 which was known to be a highly sensitive probe. We have also found that the center of observable range of calcium concentration by fluorescence lifetime measurement is lower than that by fluorescence intensity measurement.

Key words

fluorescence lifetime calcium concentration calcium green-1 oregon green 488 BAPTA-6F calcium green-5N fluo-3 fluo-4 fluo-5N 


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Copyright information

© The Optical Society of Japan 2005

Authors and Affiliations

  • Keisuke Yoshiki
    • 1
  • Hiroki Azuma
    • 1
  • Kazuhiko Yoshioka
    • 1
  • Mamoru Hashimoto
    • 1
  • Tsutomu Araki
    • 1
  1. 1.Graduate School of Engineering ScienceOsaka UniversityToyonaka, OsakaJapan

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