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Optical Review

, Volume 8, Issue 4, pp 284–286 | Cite as

Halo Reduction Technique in Phase Contrast Microscopy

  • Tatsuro OtakiEmail author
OPTICAL SYSTEMS AND TECHNOLOGIES

Abstract

A new phase contrast microscopy technique has been developed which achieves halo reduction by applying an apodization to the Zernike phase contrast method. In this technique, a new apodized phase plate is placed at the back focal plane of the objective. This plate consists of a quarter wave phase shift ring conjugated of the illuminating aperture, which has a 25#x0025; transmittance, and a pair of adjacent rings having 50#x0025; transmittance. The developed technique yields the highest resolution images without a bluish hue effect ever achieved in phase contrast microscopy.

Key words

phase contrast microscopy apodization halo phase difference bluish hue apodized phase contrast 

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Copyright information

© The Optical Society of Japan 2001

Authors and Affiliations

  1. 1.Optical Design Department, Instruments CompanyNikon CorporationShinagawa-ku, TokyoJapan

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